| 透明颤菌血红蛋白基因和λ噬菌体裂解基因在生产PHB重组大肠杆菌中的同时表达 |
| |
| 作者姓名： | 于慧敏 史悦 尹进 沈忠耀 杨胜利 |
| |
| 作者单位： | [1]DepartmentofChemicalEngineering,TsinghualJniversity,Beijing100084,China [2]ShanghaiResearchCenterofBiotechnology,AcademicSinica,Shanghai200233,China |
| |
| 摘 要： | Exogenous Vitreoscilla globin gene (vgb), lytic genes of phage γ with S amber mutation (S^-RRz) and poly(fl-hydroxybutyrate) (PHB) biosynthetic genes (phbCAB) were cloned into a same Escherichia coil cell,simultaneously or respectively. Six novel strains containing phbCAB and vgb with or without lytic genes were constructed. Strain VG1 (pTU14), in which vgb, phbCAB and S-RRz could all be successfully expressed, has superior characteristics in cell growth and PHB accumulation, while the results of strains containing vgb and phbCAB without S- RRz were not better than that of strains harbored phbCAB only. The simultaneous expression of vgb and S-RRz in the recombinant VG1 (pTU14) showed a great potential for low-cost production of PHB.
|
| 关 键 词： | 透明颤菌血红蛋白基因 γ噬菌体裂解基因 化工生产 PHB 细胞重组 大肠杆菌 同时表达 聚（β-羟基丁酸酯） 生物合成 |
| 修稿时间： | |
| 本文献已被 维普 万方数据 等数据库收录！ |
| 点击此处可从《中国化学工程学报》浏览原始摘要信息 |
|
点击此处可从《中国化学工程学报》下载免费的PDF全文 |
|
