| 基于微波消解-电感耦合等离子体质谱法(ICP-MS)测定深海鱼肌肉中12种元素 |
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| 引用本文： | 倪明龙, 邱志超, 李银花, 黄秋妹. 基于微波消解-电感耦合等离子体质谱法(ICP-MS)测定深海鱼肌肉中12种元素[J]. 食品工业科技, 2020, 41(9): 244-249. DOI: 10.13386/j.issn1002-0306.2020.09.039 |
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| 作者姓名： | 倪明龙 邱志超 李银花 黄秋妹 |
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| 作者单位： | 1. 广东食品药品职业学院, 广东广州 510520;2. 广州检验检测认证集团有限公司, 广东广州 511447 |
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| 基金项目： | 广东食品药品职业学院自然科学研究项目青年项目(2016YZ033)。广东省医学科学技术研究基金(B2018171) |
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| 摘 要： | 为了建立同时测定深海鱼中硼(B)、铝(Al)、铬(Cr)、锰(Mn)、镍(Ni)、铜(Cu)、锌(Zn)、锡(Sn)、镉(Cd)、汞(Hg)、硒(Se)、铅(Pb)12种元素含量的方法,采用HNO3-H2O2-H2O消解体系,样品经微波消解,然后利用电感耦合等离子体质谱仪(ICP-MS)测定上述元素。在碰撞模式下(KED),以内标法改善基体效应及干扰。结果表明,各元素检出限低,线性关系良好,相关系数(r)在0.9991~1.0000(Hg为0.9921),检出限为0.00074~2.2 μg/kg,定量限为0.0025~7.1 μg/kg。用该方法对生物标准物质扇贝(GBW10024)进行测定,测定结果均在证书的不确定度范围以内,对带鱼样品进行加标回收率实验,加标回收率在81.8%~110.0% 之间。通过对市场上销售的不同深海鱼进行检测分析发现,受检样品安全风险较低,4种必需微量元素含量高低顺序为Zn>Se>Cu>Cr,其中Zn含量范围为3.72~22.1 mg/kg,Se含量范围为0.162~0.391 mg/kg,Cu含量范围为0.095~0.334 mg/kg,Cr含量范围为0.003~0.054 mg/kg。该方法准确可靠,灵敏度高,适合鱼肉中多元素含量的测定,为鱼肉的质量控制提供技术支持;带鱼、大黄花鱼富含微量元素Zn,小黄花鱼富含Se,是孕妇和乳母等特殊群体比较好的食物来源。
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| 关 键 词： | 电感耦合等离子体质谱 微波消解 深海鱼 元素分析 |
| 收稿时间： | 2019-08-16 |
Determination of 12 Elements in Deep-sea Fish Muscles by ICP-MS after Pretreatment of Microwave Digestion |
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| NI Ming-long, QIU Zhi-chao, LI Yin-hua, HUANG Qiu-mei. Determination of 12 Elements in Deep-sea Fish Muscles by ICP-MS after Pretreatment of Microwave Digestion[J]. Science and Technology of Food Industry, 2020, 41(9): 244-249. DOI: 10.13386/j.issn1002-0306.2020.09.039 |
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| Authors: | NI Ming-long QIU Zhi-chao LI Yin-hua HUANG Qiu-mei |
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| Affiliation: | 1. Guangdong Food and Drug Vocational College, Guangzhou 510520, China;2. Guangjian Trusted Testing&Certification Group Co., Ltd., Guangzhou 511447, China |
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| Abstract: | In order to establish the method for Simultaneous determination of 12 elements of boron(B),aluminum(Al),chromium(Cr),manganese(Mn),nickel(Ni),copper(Cu),zinc(Zn),tin(Sn),cadmium(Cd),mercury(Hg),selenium(Se)and lead(Pb)in deep sea fish muscles,HNO3-H2O2-H2O was used to achieve the complete decomposition of the samples by microwave digestion,the elements were determined by inductively coupled plasma mass spectrometer(ICP-MS). The effects of the matrix were improved by internal standard method to reduce the interference with collision model(KED). Results showed that the detection limit of each element was low and the linear relationship was good,correlation coefficients(r)were at the range of 0.9991~1.0000(Hg was 0.9921),the detection limit was 0.00074~2.2 μg/kg,the quantitation limit was 0.0025~7.1 μg/kg.The method was successfully applied to determine the biological standard elements in scallop(GBW10024). The results were all within the uncertainty range of the certificate. Additionally,the spiked recovery rates of Trichiurus lepturus sample were between 81.8%~110.0%. Different deep-sea fishes sold in the markets were tested with this method,indicating that every fish muscle was low safety risk,the order of content of 4 essential trace elements was Zn>Se>Cu>Cr,in which the content range of Zn was 3.72~22.1 mg/kg,Se was 0.162~0.391 mg/kg,Cu was 0.095~0.334 mg/kg,and Cr was 0.003~0.054 mg/kg.The method was accurate,sensitive and reliable,and suitable for the determination of multi-element concentration in fish muscles,providing technical support for quality control of fish processing;Trichiurus lepturus and Pseudosciaena crocea were rich in Zn,and Psendosciaena polyactis was rich in Se,which were good food sources for pregnant women and nursing mothers. |
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| Keywords: | inductively coupled plasma mass spectrometry(ICP-MS) microwave digestion deep-sea fish element analysis |
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