| 两株根际促生细菌对TMV的生防作用研究 |
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| 引用本文： | 刘笑玮,秦元霞,袁莲莲,何青云,杨金广,申莉莉. 两株根际促生细菌对TMV的生防作用研究[J]. 中国烟草学报, 2018, 24(6): 82-89. DOI: 10.16472/j.chinatobacco.2018.080 |
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| 作者姓名： | 刘笑玮 秦元霞 袁莲莲 何青云 杨金广 申莉莉 |
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| 作者单位： | 1.中国农业科学院烟草研究所, 烟草行业病虫害监测与综合治理重点开放实验室, 山东省青岛市崂山区科苑经四路11号 266101 |
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| 基金项目： | 中国农业科学院基本科研业务费专项1610232016004 |
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| 摘 要： | 烟草花叶病毒（Tobacco mosaic virus，TMV）是危害烟草的主要病毒之一，其寄主范围广、侵染力和抗逆性强，尚无有效的抗病毒药剂。利用植物根际促生细菌（PGPR）对寄主的促生以及对病毒的钝化作用进行病毒病早期预防，是控制TMV的有效途径。本文对筛选到的两株PGPR菌株Sa和Sk进行了菌株鉴定及促生和防病作用研究。结果表明:Sa为芽孢杆菌属（Bacillus sp.）、Sk为假单胞菌属（Pseudomonas sp.）。在霍格兰氏营养液中添加Sa、Sk的发酵上清液，能显著促进烟苗的根系发育和茎叶生长；“半叶法”试验结果显示Sa、Sk的发酵上清液能在体外钝化TMV，并且Sa的粗蛋白溶液、去蛋白上清、蛋白失活后的粗蛋白溶液对TMV的抑制效果分别为85.2%、83.1%、36.2%，说明Sa的发酵物中对TMV起钝化作用的活性物质主要包括蛋白和非蛋白两类；Sk的粗蛋白溶液、去蛋白上清抑制TMV的效果分别为88.0%、0%，说明Sk的发酵物中对TMV起钝化作用的活性物质主要为蛋白类。利用TMV30B侵染性克隆，观察到Sa、Sk发酵上清液能显著抑制病毒的初侵染。室内栽培烟株喷施Sa、Sk发酵上清后，对TMV的防效分别为27.71%和18.55%。芽孢杆菌Sa生防效果好于Sk，具备抗病毒剂的开发潜力。
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| 关 键 词： | 植物根际促生细菌 烟草花叶病毒 生物防治 体外钝化 |
| 收稿时间： | 2018-03-28 |
Study on the bacteria effect of two PGPR biocontrol on TMV |
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| Affiliation: | 1.Key Laboratory of Tobacco Pest Monitoring Controlling & Integrated Management, Institute of Tobacco Research, Chinese Academy of Agricultural Sciences, Qingdao 266101, Shandong, China2.College of Plant Protection, Shenyang Agricultural University, Shenyang 110866, China |
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| Abstract: | Tobacco mosaic virus (TMV) is one of the main viruses that affect tobacco, with characteristics of wide host range, strong invasiveness and anti-adversity. Currently, there is few effective antiviral agent to control TMV. One feasible approach for TMV management is application of plant growth promoting rhizobacteria (PGPR), which could prevent plant from virus invading at the initial stage, as the PGPR could promote plant growth and inhibit virus directly. In this paper, two strains of PGPR, namely Sa and Sk, were identified, and the effect of growth-promoting and disease-preventing was studied. Results showed that Sa belonged to Bacillus sp. and Sk belonged to Pseudomonas sp.. Fermentation supernatants of both Sa and Sk had the effect of promoting tobacco growth and inactivating TMV in vitro. The inhibitory effects of crude protein, deproteinized supernatants and crude protein which was inactivated from Sa fermentation liquor on TMV were respectively 85.2%、83.1% and 36.2%, implying the bioactive substance of Sa was protein and non-protein element; the inhibitory effects of crude protein and deproteinized supernatants from Sa fermentation liquor on TMV were respectively 88.0% and 0%, suggesting the bioactive substance of Sk was mainly protein. By using TMV30B infectious clone tests, the passivation effect was more obvious in observation. The fermentation supernatant of both Sa and Sk could inhibit infection of TMV30B significantly. Spraying fermentation supernatant of Sa and Sk could prevent tobacco from TMV, with a respectivecontrol efficiency of 27.71% and 18.55%. In conclusion, strain Sa showed higher biocontrol effect than Sk, which indicated a huge potential for antiviral agents development. |
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