| 微杆菌ZD-M2降解二苯并噻吩的特性及其生长条件优化 |
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| 引用本文： | 张英,李伟,王妙冬,施耀. 微杆菌ZD-M2降解二苯并噻吩的特性及其生长条件优化[J]. 化工学报, 2005, 56(7): 1295-1299 |
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| 作者姓名： | 张英 李伟 王妙冬 施耀 |
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| 作者单位： | 浙江大学环境工程研究所,浙江,杭州,310027;浙江大学环境工程研究所,浙江,杭州,310027;浙江大学环境工程研究所,浙江,杭州,310027;浙江大学环境工程研究所,浙江,杭州,310027 |
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| 基金项目： | 国家自然科学基金项目（20276068）. |
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| 摘 要： | 分离得到的微杆菌(Microbacterium sp.)ZD-M2是一株专一性脱硫菌，GC-MS分析表明该菌能选择性地脱除二苯并噻吩(DBT)中的硫，沿“4S途径”代谢的终产物2-羟基联苯(2-HBP)会进一步氧化为2-甲氧基联苯(2-MBP)，同时降解产物中还有联苯（还原产物）存在.在水溶液中降解DBT的结果表明：该菌的适宜生长pH范围为6.5～9.5，最适生长温度为30 ℃，氯化铵为最佳氮源，生长的最佳浓度为1.0 g•L-1.该菌能利用多种碳源和硫源进行生长，但以甘油和DBT为最佳，最适浓度分别为2.0 g•L-1和0.2 mmol•L-1.
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| 关 键 词： | 二苯并噻吩 生物脱硫 降解途径 微杆菌 |
| 文章编号： | 0438-1157（2005）07-1295-05 |
| 收稿时间： | 2004-06-02 |
| 修稿时间： | 2004-06-02 |
Selective-desulfurization of dibenzothiophene by Microbacterium sp.ZD-M2 and optimization of growth conditions |
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| ZHANG Ying,LI Wei,WANG Miaodong,SHI Yao. Selective-desulfurization of dibenzothiophene by Microbacterium sp.ZD-M2 and optimization of growth conditions[J]. Journal of Chemical Industry and Engineering(China), 2005, 56(7): 1295-1299 |
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| Authors: | ZHANG Ying LI Wei WANG Miaodong SHI Yao |
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| Abstract: | A newly isolated strain was identified as Microbacterium sp. ZD-M2, which can specifically remove sulfur from the model compound of dibenzothiophene (DBT). Metabolites were identified by gas chromatography-mass spectrometry, and the results showed that 2-hydroxybiphenyl, the end product of the previously reported sulfur-specific pathway (also called 4S pathway), was further converted to 2-methoxybiphenyl. Biphenyl was also produced as the deoxidized metabolites. In aqueous phase the strain grew well in the pH range of 6.5—9.5.The optimal temperature for growth was 30 ℃ and the appropriate concentration of ammonium chloride as the source of nitrogen was 1.0 g•L-1. Glycerol and DBT were selected as the best carbon and sulfur sources for its growth, and the optimal concentrations were 2.0 g•L-1 and 0.2 mmol•L-1,respectively. |
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| Keywords: | dibenzothiophene biodesulfurization sulfur-specific pathway Microbacterium sp. |
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