γ‐Glu‐Met synthesised using a bacterial glutaminase as a potential inhibitor of dipeptidyl peptidase IV |
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| Authors: | Juan Yang Dongxiao Sun‐Waterhouse Chun Cui Keming Dong Mouming Zhao |
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| Affiliation: | 1. School of Food Science and Engineering, South China University of Technology, Guangzhou, China;2. Guangdong Weiwei Biotechnology Co., LTD., Guangzhou, China |
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| Abstract: | This study describes γ‐glutamyl dipeptides as competitive inhibitors of dipeptidyl peptidase‐IV (DPP‐IV), and the feasible synthesis of γ‐Glu‐Met through transpeptidation catalysed by a commercial glutaminase of Bacillus amyloliquefaciens. γ‐Glu‐Met, γ‐Glu‐Leu, γ‐Glu‐Phe, γ‐Glu‐Trp or γ‐Glu‐Tyr exhibited a competitive inhibitory effect on DPP‐IV. The yield of γ‐Glu‐Met was 26.16% under optimised conditions: 200 mm Gln, 20 mm Met, 0.1 U mL?1 enzyme, pH 9.0, 37 °C and reaction time 3 h. For the first time, the side products containing characteristic sequences, that is poly‐γ‐glutamyl short chains with a terminal Met residue (γ‐Glu‐γ‐Glu‐Met and γ‐Glu‐γ‐Glu‐γ‐Glu‐Met) were identified. The superiority of the commercial glutaminase in the synthesis of DPP‐IV‐inhibitory peptides can enable the application of this novel process for manufacturing γ‐glutamyl‐peptides as potential functional ingredients in the type 2 diabetic diet. |
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| Keywords: |
Bacillus amyloliquefaciens
DPP‐IV inhibitor enzymatic synthesis side products γ ‐Glu‐Met γ ‐glutamyl dipeptides |
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