| 利用纤维素水解液中的纤维二糖发酵制备丁二酸 |
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| 引用本文： | 徐蓉,奚永兰,张九花,戴文宇,万月佳,陈可泉,姜岷. 利用纤维素水解液中的纤维二糖发酵制备丁二酸[J]. 化工进展, 2013, 32(4): 874-877 |
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| 作者姓名： | 徐蓉 奚永兰 张九花 戴文宇 万月佳 陈可泉 姜岷 |
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| 作者单位： | 南京工业大学生物与制药工程学院，材料化学工程国家重点实验室，江苏 南京 211816 |
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| 基金项目： | 国家973计划(2009CB724701);国家863计划(2011AA02A203);国家自然科学基金(21076105);长江学者和创新团队发展计划(06-A-047);材料化学工程国家重点实验室基金;江苏省“青蓝工程”;高等学校博士学科点专项基金(20093221110005)项目 |
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| 摘 要： | 纤维素水解液中通常含有纤维二糖。本文考察了Actinobacillus succinogenes NJ113利用纤维二糖厌氧发酵生产丁二酸的能力，并利用蔗渣纤维素制备纤维二糖作为碳源用于厌氧发酵生产丁二酸。3 L发酵罐厌氧发酵结果显示：以35 g/L纤维二糖作为碳源发酵制备丁二酸，其产量为23.51 g/L，产率达到67.17%；用含有18 g/L纤维二糖和17 g/L其它糖类的蔗渣纤维素水解液作为碳源发酵制备丁二酸，丁二酸的产量和产率分别为20.00 g/L和64.73%。因此，Actinobacillus succinogenes NJ113具有较强的利用纤维二糖生产丁二酸的能力，而且利用废弃的纤维素制备纤维二糖作为碳源高效、经济地发酵制备丁二酸具有可行性。
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| 关 键 词： | 丁二酸 发酵 纤维二糖 纤维素水解液 Actinobacillus succinogenes NJ113 |
Fermentation of succinic acid using cellobiose from cellulose hydrolysates |
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| XU Rong,XI Yonglan,ZHANG Jiuhua,DAI Wenyu,WAN Yuejia,CHEN Kequan,JIANG Min. Fermentation of succinic acid using cellobiose from cellulose hydrolysates[J]. Chemical Industry and Engineering Progress, 2013, 32(4): 874-877 |
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| Authors: | XU Rong XI Yonglan ZHANG Jiuhua DAI Wenyu WAN Yuejia CHEN Kequan JIANG Min |
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| Affiliation: | State Key Laboratory of Materials-Oriented Chemical Engineering，College of Biotechnology and Pharmaceutical Engineering，Nanjing University of Technology，Nanjing 211816，Jiangsu，China |
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| Abstract: | Cellobiose was often found in cellulose hydrolysates. In this study，the ability to use cellobiose to produce succinic acid by A.succinogenes NJ113 and using cellobiose prepared from sugarcane bagasse cellulose hydrolysates as a carbon source to produce succinic acid were investigated. A final succinic acid concentration of 23.51 g/L with a yield of 67.17% was achieved from an initial cellobiose concentration of 35 g/L via batch fermentation. In batch fermentation with 18 g/L of cellobiose and 17 g/L of other sugars from sugarcane bagasse cellulose hydrolysates，a succinic acid concentration of 20.00 g/L was obtained，with a yield of 64.73%. This study suggested that A. succinogenes had a strong ability to convert cellobiose into succinic acid and cellobiose from cellulose hydrolysate could be a potential carbon source for economical and efficient succinic acid production by A. succinogenes. |
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| Keywords: | succinic acid fermentation cellobiose cellulose hydrolysate Actinobacillus succinogenes NJ113 |
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