| RS4型抗性淀粉对高脂诱导肥胖小鼠脂质代谢相关基因表达的影响 |
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| 引用本文： | 王宏伟,邬应龙. RS4型抗性淀粉对高脂诱导肥胖小鼠脂质代谢相关基因表达的影响[J]. 食品科学, 2014, 35(5): 193-198. DOI: 10.7506/spkx1002-6630-201405038 |
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| 作者姓名： | 王宏伟 邬应龙 |
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| 作者单位： | 四川农业大学食品学院，四川 雅安 625014 |
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| 基金项目： | 四川省科技支撑计划项目(2009NZ0077-007);四川农业大学“211”工程双支计划项目(2010) |
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| 摘 要： | 目的：观察不同RS4型抗性淀粉对小鼠脂质代谢及相关基因表达的影响，探讨抗性淀粉干预脂质代谢的机制。方法：选用年龄和体质量合适的雄性C57BL/6J小鼠，分别饲喂含有羟丙基交联、交联酯化、柠檬酸乙酰化甘薯淀粉3 种RS4型抗性淀粉和甘薯原淀粉的高脂饲料12 周。12 周后，测定小鼠血清指标、体质量及观察小鼠肝脏组织形态变化；并采用实时荧光定量聚合酶链式反应技术检测各组小鼠肝组织中脂肪酸合成酶（fatty acid synthese，FAS）、固醇调节元件结合蛋白-1c（sterol regulatory element binding protein-1c，SREBP-1c）、3-羟基-3-甲基戊二酰辅酶A还原酶（3-hydroxy-3-methylglutary coenzyme a reductase，HMGCR）的mRNA表达水平。结果：饲喂添加3 种RS4型抗性淀粉的高脂饲料后，高脂诱导雄性C57BL/6J肥胖小鼠体质量、血清甘油三酯水平明显降低，肝细胞脂肪变性程度明显减轻、肝脏组织中SREBP-1c mRNA表达水平明显下调；添加柠檬酸乙酰化甘薯淀粉对FAS、SREBP-1c、HMGCR 的mRNA表达水平均有不同程度的下调。结论：柠檬酸乙酰化甘薯淀粉等RS4型抗性淀粉可对高脂诱导雄性C57BL/6J肥胖小鼠的脂质代谢起到一定的干预作用，并可下调其肝脏组织中SREBP-1c mRNA等基因的表达。
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| 关 键 词： | 抗性淀粉 脂质代谢 肝脏功能 基因表达 高脂饲料 |
Effect of RS4-Type Resistant Starch on the Expression of Lipid Metabolism-Related Genes in Mice with High-Fat Diet-Induced Obesity |
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| WANG Hong-wei,WU Ying-long. Effect of RS4-Type Resistant Starch on the Expression of Lipid Metabolism-Related Genes in Mice with High-Fat Diet-Induced Obesity[J]. Food Science, 2014, 35(5): 193-198. DOI: 10.7506/spkx1002-6630-201405038 |
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| Authors: | WANG Hong-wei WU Ying-long |
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| Affiliation: | College of Food Science, Sichuan Agricultural University, Ya’an 625014, China |
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| Abstract: | Objective：To investigate the effect of different RS4-type resistant starches on the mRNA expression of lipidmetabolism-related genes in mice with high-fat (HF) diet-induced obesity and to elucidate the underlying mechanism.Methods: Weight- and age-matched male C57BL/6J mice were fed for 12 weeks on a high-fat diet containing unmodifiedsweet potato starch (SPS), hydroxypropylated and cross-linked starch (HPCL), cross-linking and octenyl succinic anhydridemodified starch (CLOSA), citric acid-treated and acetylated starch (CAAC), respectively. After the feeding period, serumlipid levels, body weight and morphological changes of liver tissue in mice were examined. Meanwhile, the mRNAexpression levels of fatty acid synthese (FAS), sterol regulatory element binding protein-1c (SREBP-1), 3-hydroxy-3-methylglutary coenzyme A reductase (HMGCR) were examined. Results: The mice fed on the RS4 diet had significantlylower body weight. The expression level of SREBP-1c mRNA was significantly lower than in those fed on either unmodifiedstarch or the HF diet. The levels of serum triglyceride and the mRNA expression levels of FAS and HMGCR were alsoreduced in mice fed on the RS4 diet. Conclusion: Owing to the alteration of the expression of related genes, the mice fedon the RS4 diet had a significantly lower hepatic lipogenesis capacity in those with HF diet-induced obesity. In conclusion,dietary supplementation with RS4-type resistant starch attenuates high fat diet-induced obesity effectively, which may beattributable to the decreased lipogenesis in the liver. |
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| Keywords: | resistant starch lipid metabolism hepatic function gene expression high-fat diet |
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