Magnetically Decorated Multiwalled Carbon Nanotubes as Dual MRI and SPECT Contrast Agents |
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| Authors: | Julie Tzu‐Wen Wang Laura Cabana Maxime Bourgognon Houmam Kafa Andrea Protti Kerrie Venner Ajay M. Shah Jane K. Sosabowski Stephen J. Mather Anna Roig Xiaoxing Ke Gustaaf Van Tendeloo Rafael T. M. de Rosales Gerard Tobias Khuloud T. Al‐Jamal |
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| Affiliation: | 1. Institute of Pharmaceutical Science, King's College London, London, UK;2. Institut de Ciència de Materials de Barcelona (ICMAB‐CSIC), Campus UAB, Bellaterra, Barcelona, Spain;3. Division of Imaging Sciences and Biomedical Engineering, King's College London, London, UK;4. Cardiovascular Division, James Black Centre, King's College London British Heart Foundation Centre of Excellence, London, UK;5. UCL Institute of Neurology, University College London, London, UK;6. Centre for Molecular Oncology, Barts Cancer Institute, Queen Mary University of London, London, UK;7. Electron Microscopy for Materials Research (EMAT), University of Antwerp, Antwerp, Belgium |
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| Abstract: | Carbon nanotubes (CNTs) are one of the most promising nanomaterials to be used in biomedicine for drug/gene delivery as well as biomedical imaging. This study develops radio‐labeled, iron oxide‐decorated multiwalled CNTs (MWNTs) as dual magnetic resonance (MR) and single photon emission computed tomography (SPECT) contrast agents. Hybrids containing different amounts of iron oxide are synthesized by in situ generation. Physicochemical characterisations reveal the presence of superparamagnetic iron oxide nanoparticles (SPION) granted the magnetic properties of the hybrids. Further comprehensive examinations including high resolution transmission electron microscopy (HRTEM), fast Fourier transform simulations, X‐ray diffraction, and X‐ray photoelectron spectroscopy assure the conformation of prepared SPION as γ‐Fe2O3. High r2 relaxivities are obtained in both phantom and in vivo MRI compared to the clinically approved SPION Endorem. The hybrids are successfully radio labeled with technetium‐99m through a functionalized bisphosphonate and enable SPECT/CT imaging and γ‐scintigraphy to quantitatively analyze the biodistribution in mice. No abnormality is found by histological examination and the presence of SPION and MWNT are identified by Perls stain and Neutral Red stain, respectively. TEM images of liver and spleen tissues show the co‐localization of SPION and MWNTs within the same intracellular vesicles, indicating the in vivo stability of the hybrids after intravenous injection. The results demonstrate the capability of the present SPION–MWNT hybrids as dual MRI and SPECT contrast agents for in vivo use. |
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| Keywords: | carbon nanotubes magnetic nanoparticles MRI SPECT imaging contrast agents |
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