Tetrahydro[5]helicene‐Based Nanoparticles for Structure‐Dependent Cell Fluorescent Imaging |
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| Authors: | Meng Li Li‐Heng Feng Hai‐Yan Lu Shu Wang Chuan‐Feng Chen |
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| Affiliation: | 1. Beijing National Laboratory for Molecular Sciences, Key Laboratory of Molecular Recognition and Function, Institute of Chemistry, Chinese Academy of Sciences, Beijing, China;2. Key Laboratory of Organic Solids, Institute of Chemistry, Chinese Academy of Sciences, Beijing, China;3. University of Chinese Academy of Sciences, Beijing, China |
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| Abstract: | Four new fluorescent dyes containing tetrahydro[5]helicene moiety characterized by three‐primary emission colors (blue‐green‐red) are designed and synthesized, and their structures are characterized by NMR, MS, and single crystal X‐ray crystallography. Organic nanoparticles based on the fluorescent dyes are then prepared by re‐precipitation method, and their photophysical properties are investigated. These nanoparticles retain the strong emissions of the organic dyes, and multicolor nanoparticles were also prepared by simply tuning the ratios of the three‐primary colors dyes. These organic nanoparticles exhibit low cytotoxicity, good photostability, and high quantum yields. Moreover, the nanoparticles can also be applied in the cell fluorescence imaging. Especially, it is interestingly found that the stained regions of these nanoparticles from membrane to cytoplasm for HeLa cells show obvious structure‐dependent properties. This strategy provides a new perspective to fluorescence probe by molecular design for specific location imaging of living cells. |
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| Keywords: | nanoparticles helicene multi‐color emission cell imaging structure‐activity relationship |
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