| 蜂胶中总酚提取纯化工艺及抗氧化活性研究 |
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| 引用本文： | 张硕，王芳，杜琳，等. 西藏蜂胶及其模拟消化液的活性成分和抗氧化活性[J]. 食品工业科技，2023，44（17）：399−405. doi: 10.13386/j.issn1002-0306.2022100181. |
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| 作者姓名： | 张硕 王芳 杜琳 潘无双 |
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| 作者单位： | 1.成都师范学院化学与生命科学学院，四川成都 611130;2.特色园艺生物资源开发与利用四川省高等学校重点实验室，四川成都 611130;3.四川农业大学食品学院，四川雅安 625014 |
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| 基金项目： | 成都师范学院校级项目（CS18ZD02）；四川省科技厅面上项目（2018JY0441）；教育部大学生创新创业训练计划支持项目（S202114389186；S202214389077）。 |
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| 摘 要： | 为研究西藏蜂胶抗氧化活性，评估开发潜力，分析了西藏蜂胶及其模拟消化液的抗氧化成分（总酚和总黄酮）和活性（还原力、羟自由基清除能力（·OH清除率）、1,1-二苯基-2-三硝基苯肼自由基清除率（DPPH·清除率）、金属离子螯合率和脂质过氧化抑制率）。结果表明，西藏蜂胶总酚和总黄酮含量分别为7.02%和10.05%。模拟消化液的总酚和总黄酮含量持续降低，模拟胃液（消化2 h）总酚和总黄酮含量分别为0.43%和6.43%，模拟肠液（消化2 h）则为0.32%和5.11%。西藏蜂胶在1 mg/mL时，还原力、·OH清除率、DPPH·清除率、金属离子螯合率和脂质过氧化抑制率达到最高值，分别为0.35、96.60%、92.53%、19.96%和97.01%。模拟胃液还原力和DPPH·清除率的最高值比未消化时升高，分别为0.39和95.69%。·OH清除率、金属离子螯合率和脂质过氧化抑制率较未消化时降低。模拟肠液·OH清除率、金属离子螯合率和脂质过氧化抑制率较模拟胃液提高，最高值分别为99.72%、69.26% 和80.69%；还原力和DPPH·清除率则降低，但DPPH·最高清除率仍有74.74%。综上所述，西藏蜂胶具有较好的体外抗氧化活性，胃肠模拟消化对抗氧化各指标均有显著影响，各指标的变化趋势不一致，但多个指标仍有较好表现。西藏蜂胶可作为一种天然抗氧化剂进行后续研发。
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| 关 键 词： | 西藏蜂胶 总酚 总黄酮 模拟消化液 抗氧化活性 |
| 收稿时间： | 2022-10-19 |
Chemical variability and pharmacological potential of propolis as a source for the development of new pharmaceutical products |
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| ZHANG Shuo, WANG Fang, DU Lin, et al. Antioxidant Components and Antioxidant Activity of Tibetan Propolis and Its Simulated Digestive Juices[J]. Science and Technology of Food Industry, 2023, 44(17): 399−405. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022100181. |
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| Authors: | ZHANG Shuo WANG Fang DU Lin PAN Wushuang |
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| Affiliation: | 1.College of Chemistry and Life Sciences, Chengdu Normal University, Chengdu 611130, China;2.Sichuan Provincial Key Laboratory for Development and Utilization of Characteristic Horticultural Biological Resources, Chengdu 611130, China;3.College of Food Science, Sichuan Agricultural University, Ya'an 625014, China |
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| Abstract: | To evaluate the antioxidant activity and potential of propolis, Tibetan propolis and its simulated digestive juice were analyzed for its chemical composition (contents of total phenolics (TPC) and total flavonoids (TFC)), reducing power and antioxidant activity (hydroxyl radical scavenging activity, 1,1-diphenyl-2-picrylhydrazyl (DPPH) assays, metal iron chelating activity and lipid peroxidation inhibition ability). The results showed that the contents of TPC and TFC in Tibetan propolis were 7.02% and 10.05%, respectively. The contents of TPC and TFC in simulated digestive juices were diminished. The contents of TPC and TFC in simulated gastric digestive juices (2 h) were 0.43% and 6.43%, respectively. The contents of TPC and TFC in simulated intestinal digestive juices (2 h) were 0.32% and 5.11%, respectively. The highest values of antioxidant activity was achieved at a concentration of 1 mg/mL, and the values of reducing power, hydroxyl radical scavenging activity, DPPH radical scavenging activity, metal iron chelating activity and lipid peroxidation inhibition ability in Tibetan propolis were 0.35, 96.60%, 92.53%, 19.96% and 97.01%, respectively. Reducing power and DPPH radical scavenging activity of simulated gastric digestive juices exhibited an increasing trend, with respect to the undigested sample. The highest values of reducing power and DPPH radical scavenging activity were 0.39 and 95.69%, respectively. Hydroxyl radical scavenging activity, metal iron chelating activity and lipid peroxidation inhibition ability of simulated gastric digestive juices exhibited a decreasing trend, with respect to the undigested sample. Simulated intestinal digestive had higher hydroxyl radical scavenging rates, metal ion chelation rates and lipid peroxidation inhibition rates than simulated gastric digestive, with highest values of 99.72%, 69.26% and 80.69%, respectively, the reducing power and DPPH radical scavenging rate experienced a decreasing tendency, but the highest DPPH radical scavenging rate remained at 74.74%. All of these suggested that Tibetan propolis had high antioxidant activity. During in vitro digestion, antioxidant indicators significantly changed, but the changing trend of each indicator was inconsistent. Several indicators still performed well. Tibetan propolis can be used as a premium natural antioxidant. |
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| Keywords: | Tibet propolis total phenolics total flavonoids simulated digestive juices antioxidant activity |
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