Electrochemical Biosensor Composed of Silver Ion‐Mediated dsDNA on Au‐Encapsulated Bi2Se3 Nanoparticles for the Detection of H2O2 Released from Breast Cancer Cells |
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| Authors: | Mohsen Mohammadniaei Jinho Yoon Taek Lee Bapurao G. Bharate Jinhee Jo Donghyun Lee Jeong‐Woo Choi |
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| Affiliation: | 1. Department of Chemical and Biomolecular Engineering, Sogang University, Mapo‐gu, Seoul, Republic of Korea;2. Department of Chemical Engineering, Kwangwoon University, Nowon‐Gu, Seoul, Republic of Korea;3. School of Integrative Engineering, Chung‐Ang University, Heukseok‐dong, Dongjak‐gu, Seoul, Republic of Korea |
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| Abstract: | A newly developed electrochemical biosensor composed of a topological insulator (TI) and metallic DNA (mDNA) is fabricated. The bismuth selenide nanoparticle (Bi2Se3 NP) is synthesized and sandwiched between the gold electrode and another Au‐deposited thin layer (Bi2Se3@Au). Then, eight‐silver‐ion mediated double‐stranded DNA (mDNA) is immobilized onto the substrate (Bi2Se3@Au‐mDNA) for the further detection of hydrogen peroxide. The Bi2Se3 NP acts as the electrochemical‐signal booster, while unprecedentedly its encapsulation by the Au thin layer keeps the TI surface states protected, improves its electrochemical‐signal stability and provides an excellent platform for the subsequent covalent immobilization of the mDNA through Au–thiol interaction. Electrochemical results show that the fabricated biosensor represents much higher Ag+ redox current (≈10 times) than those electrodes prepared without Bi2Se3@Au. The characterization of the Bi2Se3@Au‐mDNA film is confirmed by atomic force microscopy, scanning tunneling microscopy, and cyclic voltammetry. The proposed biosensor shows a dynamic range of 00.10 × 10?6m to 27.30 × 10?6m , very low detection limit (10 × 10?9m ), unique current response (1.6 s), sound H2O2 recovery in serum, and substantial capability to classify two breast cancer subtypes (MCF‐7 and MDA‐MB‐231) based on their difference in the H2O2 generation, offering potential applications in the biomedicine and pharmacology fields. |
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| Keywords: | bismuth selenide cancer cells electrochemical biosensor extracellular hydrogen peroxide topological insulators |
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