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Rapid Quantification of Live Cell Receptors Using Bioluminescence in a Flow‐Based Microfluidic Device
Authors:Cheong Fook Cheong  Hiroaki Hirata  Abdur Rub Abdur Rahman  Chwee Teck Lim
Affiliation:1. Mechanobiology Institute, 5A Engineering Drive 1, T‐Lab, #10‐01, National University of Singapore, Singapore;2. Bioelectronics Department, Institute of Microelectronics, A*STAR, Singapore;3. Department of Biomedical Engineering, 9 Engineering Drive 1, Block EA, #03‐12, National University of Singapore, Singapore
Abstract:The number of receptors expressed by cells plays an important role in controlling cell signaling events, thus determining its behaviour, state and fate. Current methods of quantifying receptors on cells are either laborious or do not maintain the cells in their native form. Here, a method integrating highly sensitive bioluminescence, high precision microfluidics and small footprint of lensfree optics is developed to quantify cell surface receptors. This method is safe to use, less laborious, and faster than the conventional radiolabelling and near field scanning methods. It is also more sensitive than fluorescence based assays and is ideal for high throughput screening. In quantifying β1 adrenergic receptors expressed on the surface of H9c2 cardiomyocytes, this method yields receptor numbers from 3.12 × 105 to 9.36 × 105 receptors/cell which are comparable with current methods. This can serve as a very good platform for rapid quantification of receptor numbers in ligand/drug binding and receptor characterization studies, which is an important part of pharmaceutical and biological research.
Keywords:gaussia luciferase  cell islands  lens‐free optics  cell surface receptors  receptor numbers  microfluidics
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