| 烟草野火病菌特异性检测引物筛选及应用 |
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| 引用本文： | 陈瑞朋,卢凯,张敏,刘元德,宗浩,牛纪军,刘文涛,徐后娟. 烟草野火病菌特异性检测引物筛选及应用[J]. 中国烟草科学, 2018, 39(1): 72-76. DOI: 10.13496/j.issn.1007-5119.2018.01.010 |
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| 作者姓名： | 陈瑞朋 卢凯 张敏 刘元德 宗浩 牛纪军 刘文涛 徐后娟 |
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| 作者单位： | 1. 山东农业大学植物保护学院, 山东 泰安 271018;2. 山东临沂烟草有限公司, 山东 临沂 276004 |
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| 基金项目： | 中国烟草总公司山东省公司面上项目"烟草细菌性叶斑病快速鉴定技术研究与应用"（201537130024070）；山东省现代农业产业技术体系创新团队项目（SDAIT-25-07） |
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| 摘 要： | 烟草野火病是山东烟区主要的细菌性病害之一，为了能够对该病害进行精确、快速的分子鉴定，本研究从NCBI基因组数据库中下载了假单胞菌属25个不同菌种和127个丁香假单胞菌属不同致病变种的全基因组数据，利用Mauve 2.3.1进行全基因组比对，获得丁香假单胞菌烟草致病变种特异性区段。在此基础上利用Primer Premier 6.0进行引物设计，筛选出了一对丁香假单胞菌烟草致病变种的特异性检测引物40429。利用7株山东不同烟区的烟草野火病菌和4株分别来自于贵州、湖北、福建及云南的烟草野火病菌进行了该引物的适用性验证，结果表明该引物均可扩增得到大小为203 bp的单一目的条带。说明该引物对于烟草野火病菌的检测具有良好的适用性，可以用于野火病菌的分子鉴定。
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| 关 键 词： | 烟草野火病 丁香假单胞菌烟草致病变种 特异性引物 分子鉴定 |
| 收稿时间： | 2017-06-20 |
Screening and Application of Pseudomonas syringae pv. Tabaci-specific Primers |
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| CHEN Ruipeng,LU Kai,ZHANG Min,LIU Yuande,ZONG Hao,NIU Jijun,LIU Wentao,XU Houjuan. Screening and Application of Pseudomonas syringae pv. Tabaci-specific Primers[J]. Chinese Tobacco Science, 2018, 39(1): 72-76. DOI: 10.13496/j.issn.1007-5119.2018.01.010 |
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| Authors: | CHEN Ruipeng LU Kai ZHANG Min LIU Yuande ZONG Hao NIU Jijun LIU Wentao XU Houjuan |
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| Affiliation: | 1. College of Plant Protection, Shandong Agricultural University, Tai'an, Shandong 271018, China;2. Shandong Linyi Tobacco Co., Ltd., Linyi, Shandong 276004, China |
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| Abstract: | Tobacco wildfire, caused by Pseudomonas syringae pv. tabaci, is an important leaf tobacco bacterial disease. In order to quickly and accurately detect this disease, we downloaded the genome sequences from NCBI, including 25 Pseudomonas genus and 127 pathovars of P. syringae, and made a blast with Mauve 2.3.1 to find the specific fragments for P. syringae pv. tabaci. Based on this result, 13 primer pairs were designed with Primer Premier 6.0 and study their specificity for P. syringae pv. tabaci. One primer pair,40429, was picked out and verified with wildfire strains of different provinces. These results showed that primer 40429 could separate P. syringae pv. tabaci from other Pseudomonas genus and other pathovars of P. syringae by a single PCR product of 203 bp. It is indicated that the primer has a good applicability to the detection of P. syringae pv. tabaci and can be used in molecular identification. |
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| Keywords: | tobacco wildfire disease Pseudomonas syringae pv. tabaci specific primers molecular detection |
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