Assessment of cell proliferation in knitting scaffolds with respect to pore‐size heterogeneity,surface wettability,and surface roughness |
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| Authors: | A Ra Jo Myoung Wha Hong Yong Sang Cho Ki Myoung Song Jun Hee Lee Dongwoo Sohn Young‐Yul Kim Young‐Sam Cho |
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| Affiliation: | 1. Division of Mechanical and Automotive Engineering, College of Engineering, Wonkwang University, Jeonbuk, Republic of Korea;2. Department of Orthopedics, Deajeon St. Mary's Hospital, Catholic University of Korea, Daejeon, Republic of Korea;3. Department of Nature‐Inspired Nano Convergence System, Nano Convergence and Manufacturing Systems Research Division, Korea Institute of Machinery and Materials (KIMM), Daejeon, Republic of Korea;4. Division of Mechanical Engineering, College of Engineering, Korea Maritime and Ocean University, Yeongdo‐gu, Busan, Republic of Korea |
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| Abstract: | In this study, various types of poly(ε‐caprolactone) (PCL) knitting scaffolds were fabricated and analyzed to assess the cell‐culturing characteristics of knitting scaffolds with respect to pore‐size heterogeneity, surface wettability, and surface roughness. First, control knitting scaffolds were fabricated using 150‐µm‐diameter PCL monofilaments. Using chloroform and NaOH, PCL knitting scaffolds with varying roughness, pore‐size heterogeneity, and surface wettability were fabricated. Cell‐culture assessments were performed on these six types of PCL knitting scaffolds. Saos‐2 cells were used for cell assessments and cultured for 14 days on each scaffold. Consequently, heterogeneous pore‐size distribution and high surface wettability were found to enhance cell proliferation in knitting scaffolds. In addition, for highly hydrophobic knitting scaffolds exhibiting water contact angles greater than 110 degrees, smaller surface roughness was found to enhance cell proliferation. According to this study, in the case of knitting scaffold, NaOH‐treated knitting scaffold, without any control for the pore‐size homogenization, could be a candidate as the optimal knitting scaffold. © 2015 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2015 , 132, 42566. |
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| Keywords: | biomedical applications biomaterials properties and characterization |
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