| 克雷伯氏杆菌发酵生产1,3-丙二醇的代谢通量优化分析 |
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| 引用本文： | 张青瑞,修志龙,曾安平. 克雷伯氏杆菌发酵生产1,3-丙二醇的代谢通量优化分析[J]. 化工学报, 2006, 57(6): 1403-1409 |
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| 作者姓名： | 张青瑞 修志龙 曾安平 |
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| 作者单位： | 大连理工大学环境与生命学院,辽宁,大连,116024;德国国家生物技术中心,德国,布伦瑞克,D-38106 |
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| 基金项目： | 科技部科技攻关计划 , 科技部科研项目 |
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| 摘 要： | 以克雷伯氏杆菌为研究对象，系统研究了1,3-丙二醇厌氧和微氧代谢途径.采用代谢通量分析法建立了代谢通量平衡模型，通过线性规划对1,3-丙二醇厌氧和微氧发酵进行了优化分析，得到其最优代谢通量分布和最大理论得率，并分析了比生长速率、底物浓度以及乙醇生成速率、氧气消耗速率、呼吸商对1,3-丙二醇得率的影响.
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| 关 键 词： | 1 3-丙二醇 代谢通量分析 线性规划 克雷伯氏杆菌 |
| 文章编号： | 0438-1157（2006）06-1403-07 |
| 收稿时间： | 2005-04-28 |
| 修稿时间： | 2005-04-282005-07-20 |
Optimization of microbial production of 1,3-propanediol by Klebsiella pneumoniae under anaerobic and microaerobic conditions by metabolic flux analysis |
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| ZHANG Qingrui,XIU Zhilong,ZENG Anping. Optimization of microbial production of 1,3-propanediol by Klebsiella pneumoniae under anaerobic and microaerobic conditions by metabolic flux analysis[J]. Journal of Chemical Industry and Engineering(China), 2006, 57(6): 1403-1409 |
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| Authors: | ZHANG Qingrui XIU Zhilong ZENG Anping |
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| Abstract: | In this paper, a simplified metabolic network was constructed according to glycerol metabolism in Klebsiella pneumoniae under anaerobic and microaerobic conditions.A model of metabolic flux balance was set up by using metabolic flux analysis.The maximum theoretical yield of 1,3-propanediol(1,3-PD)to glycerol and the optimal flux distributions were obtained by using linear programming.In addition, the influences of specific growth rates,ethanol formation rates,molar fraction of NADH2 oxidized completely by molecular oxygen in TCA (tricarboxylic acid) cycle and respiratory quotient (RQ) on the maximum theoretical yield of 1,3-PD to glycerol were analyzed as well.The metabolic flux analysis showed that the maximum theoretical yield of 1,3-PD to glycerol could reach 0.875 mol•mol-1 rather than 0.75 mol•mol-1 if all acetyl-CoA entered into TCA cycle under micoraerobic conditions instead of acetic acid pathway under anaerobic conditions.The theoretical yield of 1,3-PD decreased as the specific growth rate and the ethanol formation rate increased.There existed an optimum oxygen uptake rate and a RQ value at different specific growth rates. |
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| Keywords: | 1,3-propanediol metabolic flux analysis linear programming Klebsiella pneumoniae |
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