Phototoxic Activity and DNA Interactions of Water‐Soluble Porphyrins and Their Rhenium(I) Conjugates |
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| Authors: | Giuliana Mion Dr. Teresa Gianferrara Dr. Alberta Bergamo Prof. Gilles Gasser Dr. Vanessa Pierroz Dr. Riccardo Rubbiani Prof. Ramon Vilar Dr. Anna Leczkowska Prof. Enzo Alessio |
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| Affiliation: | 1. Department of Chemical & Pharmaceutical Sciences, Università degli Studi di Trieste, Trieste, Italy;2. Callerio Foundation Onlus, Trieste, Italy;3. Department of Chemistry, University of Zurich, Zurich, Switzerland;4. Department of Chemistry, Imperial College London, London, UK |
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| Abstract: | In the search for alternative photosensitizers for use in photodynamic therapy (PDT), herein we describe two new water‐soluble porphyrins, a neutral fourfold‐symmetric compound and a +3‐charged tris‐methylpyridinium derivative, in which either four or one [1,4,7]‐triazacyclononane (TACN) units are connected to the porphyrin macrocycle through a hydrophilic linker; we also report their corresponding tetracationic ReI conjugates. The in vitro (photo)toxic effects of the compounds toward the human cell lines HeLa (cervical cancer), H460M2 (non‐small‐cell lung carcinoma), and HBL‐100 (non‐tumorigenic epithelial cells) are reported. Three of the compounds are not cytotoxic in the dark up to 100 μm , and the fourfold‐symmetric couple revealed very good phototoxic indexes (PIs). The intracellular localization of all derivatives was studied in HeLa cells by confocal fluorescence microscopy. Although low nuclear localization was observed for some of them, it still prompted us to investigate their capacity to bind both quadruplex and duplex DNA; we observed significant selectivity in the tris‐methylpyridinium derivatives for G‐quadruplex interactions. |
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| Keywords: | antitumor agents photodynamic therapy (PDT) phototoxicity porphyrins rhenium |
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