| 高效液相色谱-串联质谱法检测动物源性食品中异丙嗪 |
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| 引用本文： | 李志刚,李慧晨,马燕红,王守伟,任南,郭文萍,郭超,李莹莹. 高效液相色谱-串联质谱法检测动物源性食品中异丙嗪[J]. 食品科学, 2019, 40(24): 320-324. DOI: 10.7506/spkx1002-6630-20190115-166 |
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| 作者姓名： | 李志刚 李慧晨 马燕红 王守伟 任南 郭文萍 郭超 李莹莹 |
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| 作者单位： | （中国肉类食品综合研究中心，北京 100068） |
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| 基金项目： | “十三五”国家重点研发计划重点专项（2017YFE0110800） |
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| 摘 要： | 建立高效液相色谱-串联质谱法测定畜肉及内脏中异丙嗪残留量的定量检测方法。首先通过甲酸-乙腈进行试样提取，然后经HLB离子交换萃取小柱净化，最后采用多反应监测模式进行定量分析。结果表明：本方法在0.5～50 ng/mL范围内线性关系良好，线性相关系数大于0.999，检出限和定量限分别为0.1 μg/kg和0.3 μg/kg；基质添加回收范围在85.7%～102.0%之间，相对标准偏差在0.9%～7.4%之间（n=6）。该方法前处理简单、快速，可用于畜肉和内脏中异丙嗪的定量测定。
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| 关 键 词： | 异丙嗪 镇静剂 高效液相色谱-串联质谱法 |
High Performance Liquid Chromatography-Tandem Mass Spectrometry Detection of Promethazine in Animal-Derived Foods |
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| LI Zhigang,LI Huichen,MA Yanhong,WANG Shouwei,REN Nan,GUO Wenping,GUO Chao,LI Yingying. High Performance Liquid Chromatography-Tandem Mass Spectrometry Detection of Promethazine in Animal-Derived Foods[J]. Food Science, 2019, 40(24): 320-324. DOI: 10.7506/spkx1002-6630-20190115-166 |
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| Authors: | LI Zhigang LI Huichen MA Yanhong WANG Shouwei REN Nan GUO Wenping GUO Chao LI Yingying |
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| Affiliation: | (China Meat Research Center, Beijing 100068, China) |
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| Abstract: | In this research, an analytical method for the determination of promethazine residues in livestock meat and viscera by high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) was established. Samples were extracted with formic acid-acetonitrile, and then the extract was purified on an HLB ion exchange extraction column before being quantitatively analyzed by using multi-reaction monitoring (MRM) mode. The results showed that the method had a good linear relationship in the concentration range of 0.5–50 ng/mL with a correlation coefficient of greater than 0.999. The limit of detection (LOD) and limit of quantitation (LOQ) were 0.1 and 0.3 μg/kg, respectively. The recoveries from spiked samples were 85.7%–102.0%, and the relative standard deviations for precision were 0.9%–7.4% (n = 6). This method is simple and rapid, and can be used for the determination of promethazine in animal-derived foods. |
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| Keywords: | promethazine sedative high performance liquid chromatography-tandem mass spectrometry |
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