Binding Characteristics and Protective Capacity of Cyanidin‐3‐Glucoside and its Aglycon to Calf Thymus DNA |
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| Authors: | Chao Zhang Xiaofei Guo Wenqian Cai Yue Ma Xiaoyan Zhao |
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| Affiliation: | Beijing Vegetable Research Center, Beijing Academy of Agriculture and Forestry Sciences, Beijing Key Laboratory of Fruits and Vegetable Storage and Processing, Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (North China), Ministry of Agriculture, Key Laboratory of Urban Agriculture (North), Ministry of Agriculture, Haidian District, Beijing, China |
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| Abstract: | The binding characteristics and protective capacity of cyanidin (Cy) and cyanidin‐3‐glucoside (C3G) to calf thymus DNA were explored for the first time. The Cy and C3G gave a bathochromic shift to the ultraviolet‐visible spectra of the DNA, indicating the formation of the DNA‐Cy and DNA‐C3G complexes. The complexes were formed by an intercalative binding mode based on the results of the fluorescence spectra and competitive binding analysis. Meanwhile, the Cy and C3G protected the DNA from the damage induced by the hydroxyl radical. The binding capacity and protective capacity of the C3G were stronger than that of the Cy. Furthermore, the formation of the DNA‐anthocyanin complexes was spontaneous when the hydrogen bond and hydrophobic force played a key role. Hence, the Cy and C3G could protect the DNA automatically from the damage induced by the hydroxyl radical. |
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| Keywords: | calf thymus DNA cyaniding cyanidin‐3‐glucoside intercalative binding mode protective capacity thermodynamics |
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