| AB-8大孔树脂分离纯化枸骨叶总黄酮的工艺研究 |
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| 引用本文： | 刘志祥,曾超珍,张 玥. AB-8大孔树脂分离纯化枸骨叶总黄酮的工艺研究[J]. 食品科学, 2010, 31(12): 76-79. DOI: 10.7506/spkx1002-6630-201012017 |
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| 作者姓名： | 刘志祥 曾超珍 张 玥 |
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| 作者单位： | 中南林业科技大学生命科学与技术学院 |
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| 基金项目： | 湖南省教育厅科学研究项目(08C920)；中南林业科技大学林木遗传育种学科资助项目(0014) |
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| 摘 要： | 以静态吸附与解吸为考察指标，利用五因素四水平的正交试验对AB-8 型大孔树脂分离纯化枸骨叶总黄酮的工艺进行研究。结果表明，AB-8 型大孔树脂对枸骨叶总黄酮有较好的纯化效果，最佳纯化工艺条件为吸附液pH4.5、吸附时间4h、解吸液体积与树脂质量之比为25:1(mL/g)、解吸液为体积分数90% 的乙醇、解吸时间2.25h，在此条件下，总黄酮回收率可达90.8%，纯度为33.4%。
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| 关 键 词： | 枸骨叶 总黄酮 AB-8 大孔树脂 分离纯化 |
| 收稿时间： | 2009-10-14 |
Using AB-8 Macroporous Resin for the Separation and Purification of Total Flavonoids from Folium Ilicis Cornutae |
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| LIU Zhi-xiang,ZENG Chao-zhen,ZHANG Yue. Using AB-8 Macroporous Resin for the Separation and Purification of Total Flavonoids from Folium Ilicis Cornutae[J]. Food Science, 2010, 31(12): 76-79. DOI: 10.7506/spkx1002-6630-201012017 |
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| Authors: | LIU Zhi-xiang ZENG Chao-zhen ZHANG Yue |
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| Affiliation: | College of Life Science and Technology, Central South University of Forestry and Technology, Changsha 410004, China |
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| Abstract: | The application of AB-8 macroporous resin for the separation and purification of crude total flavonoids extract from Folium Ilicis Cornutae was evaluated in the present study. The static adsorption-desorption process of Folium Ilicis Cornutae total flavonoids on this resin was optimized using single factor and orthogonal array design methods. The results showed that a good separation and purification of crude total flavonoids extract from Folium Ilicis Cornutae was achieved using AB-8 macroporous resin. The optimum conditions for the separation and purification of crude total flavonoids extract from Folium Ilicis Cornutae were found to be: 4 h adsorption at pH 4.5 of the sample solution, followed by 2.25 h desorption using 90% ethanol as desorption solvent added at 25-fold volume of resin weight. Under these optimum conditions, the recovery of Folium Ilicis Cornutae total flavonoids reached up to 90.8% and the purity of the final product obtained was 33.4%. |
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| Keywords: | Folium Ilicis Cornutae total flavonoids AB-8 macroporous resin separation and purification |
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