Advantages of a combined method of decalcification compared to EDTA |
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| Authors: | Vitor Aparecido Castania Joáo Walter de Souza da Silveira Ana Carolina Issy Dimitrius Leonardo Pitol Mayara Leite Castania Abel Dorigan Neto Elaine Aparecida Del Bel Helton Luiz Aparecido Defino |
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| Affiliation: | 1. Department of Biomechanics, Medicine and Rehabilitation of the Locomotor Apparatus, School of Medicine of Ribeir?o Preto, University of Sao Paulo, Ribeirao Preto/SP, Brazil;2. Department of Morphology, Physiology and Basic Pathology, School of Dentistry of Ribeir?o Preto, University of S?o Paulo, Ribeirao Preto/SP, Brazil;3. Department of Pathology and Legal Medicine, School of Medicine of Ribeir?o Preto, University of Sao Paulo, Ribeirao Preto/SP, Brazil |
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| Abstract: | Decalcification of mineralized tissues is an essential step during tissue processing in the routine histopathology. The time required for complete decalcification, and the effect of decalcifier on cellular and tissue morphology are important parameters which influence the selection of decalcifying agents. In this study, we compared a decalcifying solution (ETDA) composed of both acid and chelating agents to a classical and well‐known decalcifying agent (EDTA). To this purpose, the optic density of bone radiographs, residual calcium analysis, bone sample weight, and histological and immunohistochemical analysis were performed. Our data suggest that, similarly to EDTA, the ETDA solution completely removes the calcium ions from the samples enabling easy sectioning. However, unlike the EDTA, this agent takes much less time. Furthermore, both agents showed comparable decalcification efficacy, and similarly, they did not produce cellular, tissue or antigenicity impairments. Therefore, ETDA may be a suitable option when it is necessary an association between a rapid and complete removal of calcium minerals, and a suitable preservation of structure and antigenicity of tissues. Microsc. Res. Tech. 78:111–118, 2015. © 2014 Wiley Periodicals, Inc. |
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| Keywords: | decalcification decalcifying agent EDTA ETDA bone demineralization rat |
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