| 多形拟杆菌对大鼠血糖的影响 |
| |
| 引用本文： | 张昆南,唐英,刘世民,胡国柱,杨赟,吴晓牧. 多形拟杆菌对大鼠血糖的影响[J]. 矿产勘查, 2014, 0(9): 1-5 |
| |
| 作者姓名： | 张昆南 唐英 刘世民 胡国柱 杨赟 吴晓牧 |
| |
| 作者单位： | 1. 江西省人民医院神经内科，江西省神经病学研究所
2. 南昌大学研究生院医学部 2011 级,南昌,330006 |
| |
| 基金项目： | 江西省科技厅社会发展科技支撑项目 |
| |
| 摘 要： | 目的：探讨多形拟杆菌对大鼠血糖的影响。方法将46只 SD 大鼠按体质量的不同分为5组：普通饲料喂养组（A 组，n＝8）、高脂饲料喂养组（B 组，n＝8）、高脂饲料喂养＋多形拟杆菌菌液灌喂组（C 组，n＝8）、糖尿病模型组（D 组，n＝10）和糖尿病模型＋多形拟杆菌菌液灌喂组（E 组，n＝11）。A 组采用普通饲料喂养，B、C、D 和 E 4组采用40％脂肪和胰岛素抵抗诱导模型饲料，每只大鼠25 g·d^－1饲料喂养。适应性喂养4周后，隔夜空腹12 h，D、E 2组大鼠腹腔注射链脲佐菌素（STZ）溶液35 mg·kg^－1；A、B 和 C 3组大鼠腹腔注射0．1 mol·L^－1柠檬酸钠缓冲液。A、B 和 C 3组大鼠均造模成功，D 组死亡2只，E 组死亡3只。实验第1天开始，C、E 2组大鼠采用多形拟杆菌菌液灌胃，连续7 d；A、B 和 D 3组采用等量0．9％氯化钠注射液灌胃，连续7 d。5组大鼠第0—12周采用电子秤称体质量1次。第0、4、8和12周，使用全自动生化仪、采用己糖激酶法检测5组大鼠空腹血糖（FPG）水平，采用ELISA 法检测5组大鼠血清胰岛素水平，实时荧光定量 PCR 法测定5组大鼠肠道多形拟杆菌的数量。结果与A、B 2组比较，C 组大鼠第1—12周体质量均明显增加，D、E 2组大鼠第6—12周体质量均明显降低，C 组大鼠第8、12周 FPG 水平均显著升高，D、E 2组大鼠第4、8和12周 FPG 水平均显著升高，C、D 和 E 3组大鼠第4、8和12周血清胰岛素水平均显著升高、肠道多形拟杆菌的数量均显著增多（P ＜0．05或 P ＜0．01）。与 C 组比较，D、E 2组大鼠第1—12周体质量均明显降低，第4、8和12周 FPG 水平均显著升高、血清胰岛素水平均显著降低和肠道多形拟杆菌的数量均显著增多（P ＜0．05或 P ＜0．01）。与 D 组比较，E 组大鼠第6—12周体质量均明显增加，第4、8和12周 FPG 水平均显著升高、血清胰岛素水平均显?
|
| 关 键 词： | 糖尿病, 2型 多形拟杆菌 血糖 胰岛素 体质量 动物 实验 大鼠 |
Influence of Bacteroides Thetaiotaomicron on Plasma Glucose Levels in Rats |
| |
| ZHANG Kun-nan,TANG Ying,LIU Shi-min,HU Guo-zhu,YANG Yun,WU Xiao-mu. Influence of Bacteroides Thetaiotaomicron on Plasma Glucose Levels in Rats[J]. Mineral Exploration, 2014, 0(9): 1-5 |
| |
| Authors: | ZHANG Kun-nan TANG Ying LIU Shi-min HU Guo-zhu YANG Yun WU Xiao-mu |
| |
| Affiliation: | ZHANG Kun-nan, TANG Ying, LIU Shi-min, HU Guo-zhu, YANG Yun, WU Xiao-mu (1. Department of Neurology of J iangxi Provincial People＇s Hospital, Jiangxi Institute of Neurology, Nanchang 330006, China ; 2. 2011 Grade of Medical Department of Graduate School of Nanchang University ,Nanchang 330006,China) |
| |
| Abstract: | Objective To explore the effect of Bacteroides thetaiotaomicron（BT）on the levels of plasma glucose in rats.Methods According to different levels of body mass,46 SD rats were divided into five groups：normal diet group（group A,n=8）,high-fat diet group（group B,n=8）, high-fat diet plus BT gavage group（group C,n=8）,diabetes model group（group D,n=10）,and diabetes induction plus BT suspension gavage group（group E,n=11）.Rats were fed with ordina-ry feed in group A and with 40% fat and insulin resistance model feed in group B,C,D and E.＆amp;nbsp;Each rat was fed with feed 25 g per day.After 4 weeks of adaptive feeding and 12 hours of over-night fasting,rats were intraperitoneally injected with 35 mg·kg^-1 streptozotocin in group D and E,and with 0.1 mol·L^-1 sodium citrate buffer in group A,B and C.The rat models were suc-cessfully established.However,2 rats died in group D and 3 in group E.During the first day of ex-periment,rats in group C and E were gavaged with BT suspension intragastric administration and rats in group A,B and D with the same amount of 0.9% sodium chloride injection intragastric ad-ministration once a day for 7 days.The body mass was weighed with electronic scale once a week for 12 weeks.At weeks 0,4,8 and 12,the levels of fasting plasma glucose（FPG）were detected by hexokinase method,the levels of fasting insulin（FINS）by ELISA,and the number of intestinal tract by Real-time PCR in all the five groups.Results Compared with group A and B,body mass increased from the 1st to 12th week in group C and decreased from the 6 th to 12 th week in group D and E,levels of FPG increased at weeks 8 and 12 in group C and at weeks 4,8 and 12 in group D and E,and levels of FINS and number of BT increased at weeks 4,8 and 12 in group C,D and E （P 〈0.05 or P 〈0.01）.Compared with group C,body mass decreased from the 1st to 12 th week in group D and E,and levels of FPG and FINS and number of BT increased at weeks 4,8 and 12 in group D and E（P 〈0.05 or P 〈0.01）.Compared |
| |
| Keywords: | diabetes mellitus,type 2 Bacteroides thetaiotaomicron plasma glucose insulin body mass animals,laboratory rats |
| 本文献已被 维普 万方数据 等数据库收录！ |
|
