| 响应面法优化高产多聚谷氨酸菌株冻干保护剂的研究 |
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| 引用本文： | 陈 斐,黄天悦,张旭松,曾 鑫,郭佳强,陈孝平. 响应面法优化高产多聚谷氨酸菌株冻干保护剂的研究[J]. 武汉工程大学学报, 2020, 42(6): 610-615. DOI: 10.19843/j.cnki.CN42-1779/TQ.202008003 |
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| 作者姓名： | 陈 斐 黄天悦 张旭松 曾 鑫 郭佳强 陈孝平 |
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| 作者单位： | 武汉工程大学环境生态与生物工程学院，湖北 武汉 430205 |
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| 摘 要： | 采用实验室分离的一株高产γ-聚谷氨酸枯草芽孢杆菌（T1B）通过响应面法筛选优化高产γ-PGA菌株冻干保护剂配方。通过单因素试验，确定脱脂奶10%（质量分数，下同）、蔗糖10%、麦芽糖10%为T1B菌株冻干保护剂最优单因素。然后以细胞存活率为响应值确定了T1B菌株复合冻干保护剂的最优配方为:脱脂奶粉浓度为7.39%，蔗糖浓度为9.60%，麦芽糖浓度为10.71%。优化后的冻干保护剂在冻干过程中对于T1B菌体保护效果显著，菌体存活率的理论值最大为83.8%。并确定菌体最佳离心条件为7 000 r/min 5 min，复合冻干保护剂的最适pH为7.0，与菌体沉淀的最佳配比为2∶1，经验证此时菌体存活率为85.3%。
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| 关 键 词： | 响应面法 高产γ-聚谷氨酸枯草芽孢杆菌 冻干保护剂 真空冷冻干燥 |
Optimization of Lyophilized Protective Agent for Polyglutamic Acid Strains with High Yield by Response Surface Method |
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| CHEN Fei,HUANG Tianyue,ZHANG Xusong,ZENG Xin,GUO Jiaqiang,CHEN Xiaoping. Optimization of Lyophilized Protective Agent for Polyglutamic Acid Strains with High Yield by Response Surface Method[J]. Journal of Wuhan Institute of Chemical Technology, 2020, 42(6): 610-615. DOI: 10.19843/j.cnki.CN42-1779/TQ.202008003 |
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| Authors: | CHEN Fei HUANG Tianyue ZHANG Xusong ZENG Xin GUO Jiaqiang CHEN Xiaoping |
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| Affiliation: | School of Environmental Ecology and Bioengineering， Wuhan Institute of Technology， Wuhan 430205， China |
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| Abstract: | The formulation of a lyophilized protective agent for high-yield γ-PGA strain was optimized by response surface method using a laboratory isolated strain of high-yield γ-PGA Bacillus subtilis（T1B）. Through a single factor test， it was determined that 10% skimmed milk， 10% sucrose， and 10% maltose were the best single factors for the T1B freeze-dried protective agent. Then， based on the response value of the cell survival rate， the optimal formula of the T1B composite freeze-dried protective agent was determined: the concentration of skimmed milk powder was 7.39%， the concentration of sucrose was 9.60%， and the concentration of maltose was 10.71%. During the freeze-drying process， the optimized lyophilized protectant had a excellent performance on the bacterial protection of T1B， and the maximum theoretical value of bacterial survival rate was 83.8%. It was also determined that the best centrifugation condition of the bacteria is 7 000 r/min in 5 min， the suitable pH of the composite freeze-dried protective agent is 7.0， and the best ratio of the bacteria mud is 2∶1， and the survival rate of the bacteria is 85.3%. |
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| Keywords: | response surface method high-producing γ-polyglutamic acid bacillus subtilis freeze-drying protective additive vacuum freeze drying |
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