| 角质酶产生菌Thermobifida fusca WSH03-11诱变及高产突变株发酵条件优化 |
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| 引用本文： | 张守亮,陈坚,华兆哲,堵国成. 角质酶产生菌Thermobifida fusca WSH03-11诱变及高产突变株发酵条件优化[J]. 化工进展, 2006, 25(5): 533-537 |
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| 作者姓名： | 张守亮 陈坚 华兆哲 堵国成 |
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| 作者单位： | 江南大学,工业生物技术教育部重点实验室,无锡,214036;江南大学,生物工程学院环境生物技术室,无锡,214036;江南大学,生物工程学院环境生物技术室,无锡,214036 |
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| 基金项目： | 国家高技术研究发展计划(863计划) , 新世纪优秀人才支持计划 |
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| 摘 要： | 以Thermobifida fusca WSH03-11为出发菌株，经硫酸二乙酯诱变，获得一株遗传稳定性好的高产角质酶的突变菌株(酶活由2.3 U/mL提高为8.9 U/mL)。确定了以乙酸钠为碳源，并在2.5 L发酵罐中研究了不同pH值对突变株角质酶分批发酵的影响。根据不同pH值下发酵过程中细胞比生长速率及产物比生长速率的变化，确定了分阶段控制pH值的策略，即在发酵前20 h控制pH值7.3、20 h后控制pH值7.6，这种条件下角质酶活达到19.8 U/mL，比采用单一pH值7.0、7.3、7.6、7.9下的最大值分别提高了125%，64%，37%，47%。
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| 关 键 词： | 角质酶 诱变 pH值分段控制 细胞比生长速率 产物比生成速率 |
| 文章编号： | 1000-6613(2006)05-0533-05 |
| 修稿时间： | 2006-02-13 |
Mutagenesis of cutinase-producing strain Thermobifida fusca WSH03-11 and optimization of fermentation conditions of mutant with high cutinase production |
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| ZHANG Shouliang,CHEN Jian,HUA Zhaozhe,DU Guocheng. Mutagenesis of cutinase-producing strain Thermobifida fusca WSH03-11 and optimization of fermentation conditions of mutant with high cutinase production[J]. Chemical Industry and Engineering Progress, 2006, 25(5): 533-537 |
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| Authors: | ZHANG Shouliang CHEN Jian HUA Zhaozhe DU Guocheng |
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| Affiliation: | Key Lab of Industrial Biotechnology，Ministry of Education； Environmental Biotechnology laboratory， School of Biotechnology，Southern Yangtze University |
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| Abstract: | Thermobifida fusca WSH03-11 was mutagenized by using DES and a mutant was isolated after three runs of screening. The cutinase activity was improved from 2.3 U/mL to 8.9 U/mL. After sodium acetate was selected as fermentation carbon source,batch microbial cutinase fermentation by a mutant of Thermobifida fusca WSH03-11 at various pH values ranging from 7.0 to 7.9 was studied. Based on time courses of the specific cell growth rate and specific cutinase formation rate at different pH values,a two-stage pH value-shift strategy in which pH value was controlled at 7.3 in the first 20 h,and then switched to 7.6 after 20 h was developed. By applying this pH value-shift strategy in cutinase fermentation,the maximal cutinase activity reached 19.8 U/mL and was increased by 125%,64%,37% and 47%,compared to those with constant pH values at 7.0,7.3,7.6 and 7.9,respectively. |
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| Keywords: | cutinase mutagenesis pH value-shift strategy specific cell growth rate specific cutinase formation rate |
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