| 蛋白质离子交换的空间质量作用模型分析 |
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| 引用本文： | 陈卫东,孙彦. 蛋白质离子交换的空间质量作用模型分析[J]. 化工学报, 2002, 53(1): 88-91 |
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| 作者姓名： | 陈卫东 孙彦 |
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| 作者单位： | 天津大学化工学院生物化工系 |
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| 基金项目： | 国家杰出青年科学基金资助项目 (No .2 0 0 2 5 6 17)~~ |
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| 摘 要： | 引 言离子交换层析具有分离容量大、选择性高和介质种类多、价格适中等特点 ,是蛋白质类生物大分子分离纯化的主要手段[1] .离子交换或吸附平衡研究是离子交换层析过程设计和优化分析的基础 ,也是分离实践中评价和选择离子交换介质的重要步骤 .目前已提出多种模型用于描述离子交换平衡 ,其中应用最多的是基于Ｌａｎｇｍｕｉｒ理论的经验模型[2 ] .但是 ,Ｌａｎｇｍｕｉｒ模型不能直接反映溶液离子强度对平衡的影响 ,不利于在盐浓度梯度洗脱的离子交换层析过程中的应用 .近年来 ,基于离子交换反应和蛋白质空间屏蔽作用的空间质量作用模型受…
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| 关 键 词： | 蛋白质 离子交换平衡 空间质量作用模型 离子交换层析 分离 钝化 |
| 文章编号： | 0438-1157(2002)01-0088-04 |
| 修稿时间： | 2000-11-15 |
ANALYSIS OF STERIC MASS-ACTION MODEL FOR PROTEIN ION-EXCHANGE EQUILIBRIUM |
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| CHEN Weidong,SUN Yan. ANALYSIS OF STERIC MASS-ACTION MODEL FOR PROTEIN ION-EXCHANGE EQUILIBRIUM[J]. Journal of Chemical Industry and Engineering(China), 2002, 53(1): 88-91 |
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| Authors: | CHEN Weidong SUN Yan |
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| Abstract: | The ion-exchange equilibrium of bovine serum albumin (BSA) on an anion exchanger was studied.The usefulness of steric mass-action (SMA) model was analyzed for describing protein ion-exchange equilibrium in different buffer systems.The results showed that the SMA model was useful in the prediction of protein adsorption decrease with increasing ionic strength in a wide pH range.However,at higher salt concentrations where very low protein adsorption was achieved,the model predicted adsorption capacity was higher than the experimental results.At lower pH close to the isoelectric point of BSA,the accuracy of the model prediction further decreased.It is considered that the SMA model could be further improved by taking into account the factors influencing the three-dimensional structure of proteins (e.g., pH) and the effect of salt concentration on the intermolecular interactions of proteins. |
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| Keywords: | protein ion-exchange equilibrium steric mass-action model chromatography |
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