| 高效液相色谱法测定马铃薯块茎的绿原酸含量 |
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| 引用本文： | 张建华,金黎平,谢开云,屈冬玉. 高效液相色谱法测定马铃薯块茎的绿原酸含量[J]. 食品科学, 2007, 28(5): 301-304 |
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| 作者姓名： | 张建华 金黎平 谢开云 屈冬玉 |
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| 作者单位： | 中国农业科学院蔬菜花卉研究所； 中国农业科学院蔬菜花卉研究所 北京100081； 北京100081； |
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| 基金项目： | 国家高技术研究发展计划(863计划) |
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| 摘 要： | 目的:建立测定马铃薯块茎中绿原酸含量的高效液相色谱方法,并测定不同品质特点的马铃薯的绿原酸含量。方法:对甲醇浓度、固液比和超声提取时间三个试验因素进行正交试验设计:ZOBAX C18色谱柱,250mm×4．6mm 5μm;流动相:水-甲醇-冰醋酸（20∶80∶2;30∶70∶2;40∶60∶2）;甲醇-0．4%磷酸溶液（10∶90）:流速: 1．0ml/min;柱温:室温;进样体积:5μl;检测波长为327nm。结果:精密度:RSD为3．2%,n=6,加样回收率为97．5%,提取条件为:甲醇浓度70%,固液比1∶100,超声提取时间40min。确定水∶甲醇∶冰醋酸=30∶70∶2为流动相。
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| 关 键 词： | 高效液相色谱法 马铃薯 块茎 绿原酸 |
| 文章编号： | 1002-6630（2007）05-0301-04 |
| 修稿时间： | 2006-06-26 |
Determination of Chlorogenic Acid of Potato Tuber by HPLC |
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| ZHANG Jian-hua,JIN Li-ping,XIE Kai-yun,QU Dong-yu. Determination of Chlorogenic Acid of Potato Tuber by HPLC[J]. Food Science, 2007, 28(5): 301-304 |
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| Authors: | ZHANG Jian-hua JIN Li-ping XIE Kai-yun QU Dong-yu |
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| Affiliation: | Institute of Vegetable and Flower,Chinese Academy of Agricultural Sciences,Beijing 100081,China |
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| Abstract: | Object:To establish a new HPLC method for determination of chlorogenic acid（CGA）in potato,and determine its content of CGA in five cultivars.Method:Orthogonal experiment for three factors:CH3OH concentration,ratio of solid and liquid and time of ultrasounic extraction.CGA was analyzed with C18column（250 mm×4.6 mm,5μm）and with detector at 327 nm.The mobile phase consisting HO-CH3OH-CH3COOH（20:80:2;30:70:2;40:60:2 respectively）,CH3OH-0.4% H3PO4 （10:90）.Results:The recovery of CGA is 97.5%,precision（RSD）3.2%（n=6）;CGA extraction conditions are:70% CH3OH,ratio of solid and liquid=1:100,and ultrasounic extraction time 40 min with determined mobile phase H2O-CH3OH-CH3COOH=30: 70:2. |
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| Keywords: | HPLC potato tuber chlorogenic acid |
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