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乳酸钠对铜绿假单胞菌生长的影响
引用本文:熊成,董庆利,姚远. 乳酸钠对铜绿假单胞菌生长的影响[J]. 食品科学, 2012, 33(13): 144-147. DOI: 10.7506/spkx1002-6630-201213030
作者姓名:熊成  董庆利  姚远
作者单位:上海理工大学医疗器械与食品学院
基金项目:国家自然科学基金项目(308008640);上海市研究生创新基金项目(JWCXSL1002)
摘    要:为探讨乳酸钠对铜绿假单胞菌(Pseudomonas aeruginosa)的特定抑制作用,揭示乳酸钠质量浓度与介质pH值水平影响乳酸钠抑菌效果的规律,以营养肉汤培养基为介质,按照乳酸钠质量浓度(0、0.2、0.5、1.0、1.5、2.0g/100mL)单因素试验及乳酸质量浓度与介质pH值(4.0~6.2)的两因素交互试验设计,25℃恒温培养。结果表明:在适当的pH值范围内,乳酸钠(NaL)表现出良好的抑菌效果:当介质pH值小于NaL质量浓度的抑菌临界pH值时,NaL抑菌效果与其质量浓度成正比,介质pH4.8,抑菌效果2.0g/100mL NaL>1.0g/100mL NaL>0.5g/100mL NaL。NaL质量浓度一定,其抑菌效果与介质pH值成反比。

关 键 词:防腐剂  铜绿假单胞菌  乳酸钠  pH值  

Effect of Sodium Lactate on the Growth of Pseudomonas aeruginosa
XIONG Cheng,DONG Qing-li,YAO Yuan. Effect of Sodium Lactate on the Growth of Pseudomonas aeruginosa[J]. Food Science, 2012, 33(13): 144-147. DOI: 10.7506/spkx1002-6630-201213030
Authors:XIONG Cheng  DONG Qing-li  YAO Yuan
Affiliation:(School of Medical Instrument and Food Engineering, University of Shanghai for Science and Technology, Shanghai 200093, China)
Abstract:Nutrient broth media containing different concentrations of sodium lactate(0,0.2,0.5,1.0,1.5 g/100 mL and 2.0 g/100 mL;consequent pH 4.0-6.2) were used to culture Pseudomonas aeruginosa constantly at 25 ℃ to examine the inhibitory effect of sodium lactate on bacterial growth.In the proper pH range,sodium lactate showed outstanding antibacterial activity.At a medium pH level lower than the critical inhibitory pH,the antibacterial effect of sodium lactate was directly proportional to the corresponding concentration.At a medium pH of 4.8,sodium lactate exhibited the highest antibacterial activity at 2.0 g/100 mL,followed by 1.0 g/100 mL;the antibacterial activity was the weakest at 0.5 g/100 mL.At a constant sodium lactate concentration,the antibacterial activity was inversely proportional to medium pH.
Keywords:preservative  Pseudomonas aeruginosa  sodium lactate  pH
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