| 鉴别烟草属植物的特异PCR引物研究 |
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| 引用本文： | 童治军,张轲,孙浩巍,张晓伟,方敦煌,曾建敏,陈学军,陈丹,龙杰,张冀武,蔡洁云,王春琼,肖炳光. 鉴别烟草属植物的特异PCR引物研究[J]. 中国烟草学报, 2022, 28(2): 101-110. DOI: 10.16472/j.chinatobacco.2021.T0024 |
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| 作者姓名： | 童治军 张轲 孙浩巍 张晓伟 方敦煌 曾建敏 陈学军 陈丹 龙杰 张冀武 蔡洁云 王春琼 肖炳光 |
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| 作者单位： | 1.云南省烟草农业科学研究院/烟草行业烟草生物技术育种重点实验室/国家烟草基因工程研究中心，云南昆明 650021 |
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| 基金项目： | 中国烟草总公司云南省公司项目2020530000241034“烟草抗黑胫病基因聚合与抗黑胫病烤烟新品系选育”2020530000241009“基于拉曼光谱的烟草农药残留检测技术研发”2020530000241037 |
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| 摘 要： | [目的]为科学、高效鉴别检测涉案制烟原料中是否含有烟草材料.[方法]利用公共数据库中烟碱代谢途径相关基因序列和茄科基因组数据(含烟草未公开数据)开发分子标记,通过对3个烟草亚属11个组的91份烟草材料和17份非烟草材料进行实验验证,获得烟草属特异性扩增分子标记.[结果]从209对新开发的引物中筛选出2对稳定、可靠且具有...
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| 关 键 词： | 涉案制烟原料 烟碱 烟草属特异性标记 |
| 收稿时间： | 2021-02-25 |
Study on specific PCR primers for identification of Nicotiana plants |
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| Affiliation: | 1.Yunnan Academy of Tobacco Agricultural Sciences / Key Laboratory of Tobacco Biotechnological Breeding / National Tobacco Genetic Engineering Research Center, Kunming 650021, China2.Yunnan Tobacco Quality Inspection & Supervision Station, Kunming 650106, China |
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| Abstract: | To scientifically and efficiently identify and detect the tobacco materials contained in the tobacco raw materials involved in the case, molecular markers were developed using the sequence data of genes related to nicotine metabolism pathway and genomic data of Solanaceae (including unpublished tobacco genomic data) in public database in this study. The Nicotiana-specific markers were obtained by experimentally verifying 17 non-tobacco materials and 91 tobacco materials belonging to 11 sections of 3 sub-genus. The results of the study showed that 2 pairs of stable, reliable and Nicotiana-specific primers were screened out from 209 newly developed markers, which could clearly amplify the target length fragments in 91 Nicotiana materials, while there was no PCR product amplification in 17 non-Nicotiana materials. The method is simple and easy to operate, so it can be applied to the authenticity determination of tobacco materials in the anti-counterfeiting and anti-smuggling work of tobacco industry. |
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