13-Hydroxy-9Z,15Z-Octadecadienoic Acid Production by Recombinant Cells Expressing Lactobacillus acidophilus 13-Hydratase |
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| Authors: | Woo-Ri Kang Chul-Soon Park Kyung-Chul Shin Kyoung-Rok Kim Deok-Kun Oh |
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| Affiliation: | , Department of Bioscience and Biotechnology, Konkuk University, Seoul, South Korea |
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| Abstract: | Recombinant Escherichia coli cells expressing linoleate 13‐hydratase from Lactobacillus acidophilus were permeabilized by treating with 0.2 M NaCl. The optimal conditions for the production of 13‐hydroxy‐9,15(Z,Z)‐octadecadienoic acid (13‐HODE) from α‐linolenic acid by permeabilized recombinant cells were pH 6.0, 40 °C, 7.5 % (v/v) methanol, 60 g/l permeabilized cells, and 15 g/l α‐linolenic acid. Under these conditions, permeabilized cells produced 7.5 g/l 13‐HODE after 6 h, with a conversion yield of 50 % (w/w) and a volumetric productivity of 1.25 g/l/h. These values were 161 and 160 % of those obtained by nonpermeabilized cells, respectively. To the best of our knowledge, this is the first report on the process optimization for the biotechnological production of 13‐HODE. |
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| Keywords: | Cell permeabilization Lactobacillus acidophilus 13‐Hydroxy‐9,15(Z,Z)‐octadecadienoic acid Linoleate 13‐hydratase α‐Linolenic acid |
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