| 三种荧光分光光度法测定肿瘤组织中阿霉素含量的研究 |
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| 引用本文： | 臧凯宏,张宝来,王维波,马建秀,鞠洋,高明堂,李文广,吴勇杰. 三种荧光分光光度法测定肿瘤组织中阿霉素含量的研究[J]. 金属学报, 2005, 10(1): 69-74 |
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| 作者姓名： | 臧凯宏 张宝来 王维波 马建秀 鞠洋 高明堂 李文广 吴勇杰 |
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| 作者单位： | 兰州大学药学院, 甘肃省中药新药临床前研究重点实验室, 兰州 730000, 甘肃 |
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| 摘 要： | 目的: 通过比较测定肿瘤组织中阿霉素(ADR)含量的3 种荧光分光光度法, 建立简便、快捷的测定肿瘤组织中阿霉素含量的荧光分光光法。方法: 分别采用正丁醇法、硝酸银法、酸性异丙醇法测定水溶液及肿瘤组织匀浆中ADR 的含量, 比较荧光光谱曲线、回收率和灵敏度。结果: 硝酸银法测定瘤组织匀浆中的ADR 含量, 背景荧光值低, 线性范围0.2～0.8 mg·L-1, 最低检出量0.15 mg·L-1, 回收率超过97 %以上。酸性异丙醇法与正丁醇法测定瘤组织中的ADR 含量, 背景荧光值高, 回收率分别<40 %和50 %。结论: 硝酸银法适合肿瘤组织中的ADR 的含量测定, 该方法准确、灵敏、可靠。
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| 关 键 词： | 阿霉素 荧光分光光度法 肿瘤组织 含量测定 |
| 收稿时间： | 2004-10-15 |
| 修稿时间： | 2004-11-28 |
Research on three fluorospectrophotometry methods for determination of concentration of adriamycin in tumor tissue |
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| ZANG Kai-hong,ZHANG Bao-lai,WANG Wei-bo,MA Jian-xiou,JU Yang,GAO Ming-tang,LI Wen-guang,WU Yong-jie. Research on three fluorospectrophotometry methods for determination of concentration of adriamycin in tumor tissue[J]. Acta Metallurgica Sinica, 2005, 10(1): 69-74 |
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| Authors: | ZANG Kai-hong ZHANG Bao-lai WANG Wei-bo MA Jian-xiou JU Yang GAO Ming-tang LI Wen-guang WU Yong-jie |
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| Affiliation: | College of Pharmaceutical Science of Lanzhou University, Key Laboratory of Preclinical Study for New Traditional Chinese Medicine of Gansu Province, Lanzhou 730000, Gansu, China |
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| Abstract: | AIM: To establish a simple and rapid fluorospectrophotometry method for the determination of concentration of adriamycin (ADR)in tumor tissue by comparing with three fluorospectrophotometry methods. METHODS: The concentrations of ADR in water and in tumor tissue were measured by three fluorospectrophotometry methods: n-butanol, silver nitrate and acidityisopropanol assay.The fluorescence spectrum curve, recovery and sensitivity were compared.RESULTS: The method of silver nitrate for determination of concentration of ADR in tumor tissue had low background tissue fluorescence.The linear range was 0.2-0.8 mg·L-1, the limit of detection was 0 .15 mg·L-1, and the recovery was over 97% by silver nitrate method .The method of acidity- isopropanol and n-butanol for determination of ADR in tumor-tissue fluorescence and low recovery which was <40 % by acidity-isopropanol assay and <50 % by n-butanol assay, respectively.CONCLUSION: The method of silver nitrate shows accurate, sensitive and reliable characterization for determination of concentration of ADR in tumor tissue. |
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| Keywords: | adriamycin fluorospectrophotometry tumor tissue assay |
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