Enzymatic and molecular characteristics of Geotrichum candidum strains as a starter culture for malting |
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| Authors: | Michał Piegza Danuta Witkowska Regina Stempniewicz |
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| Affiliation: | Department of Biotechnology and Food Microbiology, Wroc?aw University of Environmental and Life Sciences, , 51‐630 Wroclaw, Poland |
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| Abstract: | Geotrichum candidum yeasts are proposed as a starter culture during malting. They have a double positive effect on the process. They eliminate the fungal pathogenic microflora and improve the technological properties of the finished product, malt. Among published research little or no information can be found considering the biosynthesis of hydrolytic enzymes. Most of the data is related to the production of lipases, proteases and cellulases. This paper examines the enzymology of a number of G. candidum strains. The main focus within the evaluation process was placed on whole cell barley grain cultivation. Hydrolase, which is present in cell wall degradation, was found at a satisfactory level. All tested strains produced both β‐1,3‐glucanases and β‐1,4‐glucanases capable of hydrolysing barley β‐glucan and reducing the amount of this polysaccharide in the wort. Molecular analysis of the tested strains with the use of restriction fragment length polymorphism–polymerase chain reaction and randomly amplified polymorphic DNA confirmed not only the species affiliation, but also the genetic similarity between the tested strains. Copyright © 2014 The Institute of Brewing & Distilling |
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| Keywords: | Geotrichum candidum malting hydrolases chitinases RFLP‐PCR RAPD |
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