| 一株反硝化细菌LZ-14的筛选及其脱氮特性 |
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| 引用本文： | 陈朋,胡文容,裴海燕. 一株反硝化细菌LZ-14的筛选及其脱氮特性[J]. 山东大学学报(工学版), 2009, 39(5): 133-138 |
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| 作者姓名： | 陈朋 胡文容 裴海燕 |
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| 作者单位： | 山东大学环境科学与工程学院, 山东 济南 250100; 山东省环境科学工程技术研究中心, 山东 济南 250061 |
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| 基金项目： | 国家重点基础研究发展计划(973计划)资助项目(2007CB416606);;山东省环保局资助项目[2006]034;;山东省环保局资助项目(hcyf0602) |
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| 摘 要： | 采用稀释土壤悬液-涂布平板法从芦竹根际土壤中分离出22株具有硝酸盐还原能力的细菌,从中筛选出一株产气快、脱氮率高的反硝化细菌LZ 14.该菌株为革兰氏阴性菌,菌体短杆状,大小2.0μm×0.5μm,兼性厌氧,可运动.以乙酸钠为碳源、硝酸钾为氮源分别考察了n(C)／n(N)和pH值对菌株LZ-14生长及脱氮过程的影响.结果表明最佳n(C)／n(N)为5,在n(C)／n(N)为5,初始硝态氮质量浓度为150?mg／L的情况下,36?h内可将硝态氮完全去除,TN去除率可达到84.5％.最适pH值范围在7～8之间,此区间以外,菌株LZ-14难以生长及保持脱氮能力.菌株LZ-14的脱氮过程主要发生在菌体生长的第12～36?h,并伴随有一定量亚硝酸盐的累积,随后24?h内亚硝酸盐可被完全降解.
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| 关 键 词： | 反硝化细菌 筛选 碳氮比 pH 生物脱氮, |
| 收稿时间： | 2008-10-31 |
Screening of a denitrifying bacterium strain LZ-14 and its nitrogen removal characteristics |
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| CHEN Peng,HU Wen-Rong,FEI Hai-Yan. Screening of a denitrifying bacterium strain LZ-14 and its nitrogen removal characteristics[J]. Journal of Shandong University of Technology, 2009, 39(5): 133-138 |
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| Authors: | CHEN Peng HU Wen-Rong FEI Hai-Yan |
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| Affiliation: | School of Environmental Science and Engineering, Shandong University, Jinan 250100, China; Shandong Provincial Engineering Centre on Environmental Science and Technology, Jinan 250100, China |
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| Abstract: | 22 nitrate reducing strains were isolated using dilute soil suspension-spread plate techniques from soil that sampled from the rhizosphere of Arundo donax L.. Denitrifying bacterium strain LZ-14 with faster gas producing and higher nitrogen removal efficiency was screened. The cells of strain LZ-14 were Gram-negative,facultative anaerobic,mobile and short rod-shaped. The effects of the C/N ratio and pH on the growth and denitrification process of strain LZ-14 were respectively investigated with acetate and ... |
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| Keywords: | denitrifying bacteria screen n(C)／n(N) pH biological nitrogen removal |
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