| 高速逆流色谱法分离纯化内吗啡肽-1 |
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| 引用本文： | 许旭旭,毛政,吴斌,孙洪林,何冰芳. 高速逆流色谱法分离纯化内吗啡肽-1[J]. 化工进展, 2011, 30(9): 2064 |
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| 作者姓名： | 许旭旭 毛政 吴斌 孙洪林 何冰芳 |
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| 作者单位： | 1.南京工业大学生物与制药工程学院，江苏 南京 210009；2浙江省食品药物监督管理局培训中心，浙江 杭州 310012 |
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| 基金项目： | 国家973计划项目(2011CB710800) |
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| 摘 要： | 利用高速逆流色谱技术对化学酶法合成的内吗啡肽-1粗样进行分离。分析了内吗啡肽-1粗样杂质的分配系数，选择乙酸乙酯-甲醇-水（3∶1.5∶3，体积比）作为两相溶剂系统，上相为固定相，下相为流动相，在主机转速为900 r/min，流速为1.0 mL/min，检测波长为280 nm条件下分离制备。从120 mg粗样中分离得到71 mg样品，内吗啡肽-1的纯度达99.1%，样品回收率为91.5%。该方法简便、快速，为高纯度内吗啡肽-1少量制法提供了技术，并可为高速逆流分离小肽合成产物提供指导。
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| 关 键 词： | 高速逆流色谱 分离纯化 内吗啡肽-1 |
Preparative isolation and purification of endormophin-1 by high-speed counter-current chromatography |
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| XU Xuxu,MAO Zheng,WU Bin,SUN Honglin,HE Bingfang. Preparative isolation and purification of endormophin-1 by high-speed counter-current chromatography[J]. Chemical Industry and Engineering Progress, 2011, 30(9): 2064 |
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| Authors: | XU Xuxu MAO Zheng WU Bin SUN Honglin HE Bingfang |
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| Affiliation: | 1College of Biotechnology and Pharmaceutical Engineering，Nanjing University of Technology，Nanjing 210009，Jiangsu，China；2Zhejiang Food and Drug Administration Training Center，Hangzhou 310012，Zhejiang，China |
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| Abstract: | Endormophin-1 synthesized by chem-biological method was purified by high-speed counter-current chromatograph technology（HSCCC）. The optimum separation conditions were as follows：A two-phase solvent system was ethyl acetate-methanol-water（3∶1.5∶3，v/v/v）. The lower phase as the mobile phase was operated at a flow rate of 1.0 mL /min，while the apparatus was rotated at 900 r/min，and detection were length was at 280 nm. Each time 120 mg of the crude sample was loaded under these conditions，and 71 mg of endorphin-1 with the purity of 99.1% was obtained. The recovery was 91.5%. The method was relatively simple，fast，and applicable to the large-scale isolation and separation of endormophin-1. |
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| Keywords: | high-speed counter-current chromatography(HSCCC) preparative isolation and purification endormophin-1 |
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