| 双波长比值光谱法测定饮料中的色素 |
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| 引用本文： | 杜建中,李青梅,朱艺强,丁 玎,谭永辉. 双波长比值光谱法测定饮料中的色素[J]. 食品科学, 2010, 31(10): 259-262. DOI: 10.7506/spkx1002-6630-201010056 |
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| 作者姓名： | 杜建中 李青梅 朱艺强 丁 玎 谭永辉 |
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| 作者单位： | 1.湛江师范学院 广东高校新材料工程技术开发中心 2.圣地亚哥州立大学公共卫生学院 3.加州大学圣地亚哥分校预防医学系 |
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| 基金项目： | 湛江师范学院科学研究基金资助项目(L0514) |
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| 摘 要： | 应用双波长比值光谱法测定了果味汽水、水果泡泡中胭脂红、日落黄、酒石黄的含量。依据该3 种色素的比值光谱特征，以日落黄为干扰组分，分别选择431、456nm 和509、525nm 为测定酒石黄和胭脂红的波长；以胭脂红为干扰组分时，选择514、535nm 为测定日落黄的波长。结果显示，酒石黄在0.1～60mg/L、胭脂红在0.2～80mg/L、日落黄在0.8～90mg/L 的质量浓度范围内线性关系良好，平均回收率分别是100.5%、101.1%和102.4%。本方法操作简便、易于推广，可应用于实际样品的检测。
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| 关 键 词： | 双波长比值法 胭脂红 日落黄 酒石黄 饮料 |
| 收稿时间： | 2009-08-17 |
Dual-wavelength Ratio Spectrometric Determination of Carmine,Sunset Yellow and Tartrazine in Beverages |
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| DU Jian-zhong,LI Qing-mei,ZHU Yi-qiang,DING Ding,TAN Yong-hui. Dual-wavelength Ratio Spectrometric Determination of Carmine,Sunset Yellow and Tartrazine in Beverages[J]. Food Science, 2010, 31(10): 259-262. DOI: 10.7506/spkx1002-6630-201010056 |
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| Authors: | DU Jian-zhong LI Qing-mei ZHU Yi-qiang DING Ding TAN Yong-hui |
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| Affiliation: | 1. Development Center for New Materials Engineering and Technology in Universities of Guangdong, Zhanjiang Normal University,Zhanjiang 524048, China；2. Graduate School of Public Health, San Diego State University, San Diego 92123, USA；3. Department of Family and Preventive Medicine, University of California, San Diego, San Diego 92137, USA |
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| Abstract: | A dual-wavelength ratio spectrometric method was presented for the determination of carmine, sunset yellow and tartrazine in fruit-flavored carbonated beverages. According to the spectral characteristics of the pigments, the wavelengths were chosen to be 431 nm and 456 nm for tartrazine detection and 509 nm and 525nm for carmine detection under the disturbance of sunset yellow and 514 nm and 535 nm for sunset yellow detection under the disturbance of carmine. Results showed that the developed standard curves for determining carmine, sunset yellow and tartrazine all exhibited good linearity over the concentration ranges of 0.2 to 80, 0.8 to 90 mg/L and 0.1 to 60 mg/L, respectively. The mean recoveries of 5 replicates of carmine, sunset yellow and tartrazine in orange-flavored Miranda soft drink were 100.5%, 101.1% and 102.4% respectively. This method proved to be simple and convenient and thus deserves to be popularized. |
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| Keywords: | dual-wavelength ratio carmine sunset yellow tartrazine bevevage |
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