| 万寿菊花中反式叶黄素提取皂化工艺的优化 |
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| 引用本文： | 王 闯,李大婧,宋江峰,刘春泉. 万寿菊花中反式叶黄素提取皂化工艺的优化[J]. 食品科学, 2010, 31(24): 95-101. DOI: 10.7506/spkx1002-6630-201024020 |
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| 作者姓名： | 王 闯 李大婧 宋江峰 刘春泉 |
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| 作者单位： | 1. 东北林业大学林学院2. 江苏省农业科学院农产品加工研究所3. 国家农业科技华东(江苏)创新中心农产品加工工程技术研究中心 |
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| 基金项目： | 江苏省自然科学基金项目(BK2010467)；中央高校基本科研业务费专项(DL10BA12) |
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| 摘 要： | 以万寿菊干花颗粒为原料，对万寿菊花中反式叶黄素进行同时提取皂化工艺研究。考察不同有机溶剂和用量、KOH- 乙醇溶液质量浓度和用量、提取皂化温度和时间等因素对反式叶黄素提取皂化效果的影响。在单因素试验基础上，采用响应曲面法对影响万寿菊花中反式叶黄素提取皂化效果的3 个主要因素即石油醚用量、KOH-乙醇溶液质量浓度和提取皂化温度进行优化，建立并分析各因素与反式叶黄素得率关系的数学模型，同时对反式叶黄素粗品进一步分离纯化。结果表明：采用有机溶剂法进行同时提取皂化的最佳工艺条件为石油醚用量42.6mL/g、温度58.8℃、KOH- 乙醇溶液质量浓度0.099g/mL，在此条件下反式叶黄素得率为1.499%。对反式叶黄素粗品进一步纯化，最终获得纯度达90.42% 的反式叶黄素晶体。
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| 关 键 词： | 万寿菊 反式叶黄素 提取皂化 响应曲面法 纯化 |
| 收稿时间： | 2010-03-02 |
Extraction and Saponification of Trans-lutein from Marigold Flowers |
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| WANG Chuang,LI Da-jing,SONG Jiang-feng,LIU Chun-quan. Extraction and Saponification of Trans-lutein from Marigold Flowers[J]. Food Science, 2010, 31(24): 95-101. DOI: 10.7506/spkx1002-6630-201024020 |
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| Authors: | WANG Chuang LI Da-jing SONG Jiang-feng LIU Chun-quan |
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| Affiliation: | 1. College of Forestry, Northeast Forestry University, Harbin 150040, China；2. Institute of Farm Products Processing, JiangsuAcademy of Agricultural Sciences, Nanjing 210014, China；3. Engineering Research Center for Agricultural Products Processing,National Agricultural Science and Technology Innovation Center in East China, Nanjing 210014, China |
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| Abstract: | Marigold (Tagetes erecta) flowers were used as raw materials to investigate the extraction and saponification of translutenin from marigold flowers. The effects of organic solvent amount, KOH-ethanol concentration and amount, extraction and saponification temperature and time on extraction and saponification rate of trans-lutenin from marigold flowers was explored. Based on the single factor experimental results, response surface experiments were used to optimize the extraction and saponification efficiency of trans-lutein and a mathematic model between the factors and extraction rate of trans-lutenin from marigold flowers was established. Meanwhile, crude trans-lutein was further purified. Results indicated that the optimal extraction parameters were raw material amount of 1.00 g, petroleum ether amount of 42.6 mL/g, KOH-ethanol concentration of 0.099 g/mL and extraction temperature of 58.8 ℃. Under the optimal extraction conditions, the extraction rate of trans-lutein was up to 1.499%. After the purification of trans-lutein, the content of trans-lutein crystal was up to 90.42%. |
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| Keywords: | Tagetes erecta trans-lutein extraction and saponification response surface methodology purification |
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