| 黄粉虫抗氧化活性肽的分离纯化研究 |
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| 引用本文： | 张莉莎,张建新,欧晓锋,张立佳. 黄粉虫抗氧化活性肽的分离纯化研究[J]. 食品科学, 2009, 30(22): 180-183. DOI: 10.7506/spkx1002-6300-200922040 |
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| 作者姓名： | 张莉莎 张建新 欧晓锋 张立佳 |
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| 作者单位： | 西北农林科技大学食品科学与工程学院 |
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| 摘 要： | 为制备具有抗氧化活性的黄粉虫生物活性肽，采用双酶水解黄粉虫蛋白粉，对酶解产物进行葡聚糖SephadexG-25 凝胶柱层析和阳阴离子交换柱层析分离纯化，并对其各组分分子量分布及其抗氧化性进行研究，对抗氧化活性最佳的肽段进行氨基酸组成分析。结果显示，纯化得到的抗氧化多肽对羟自由基和超氧阴离子自由基具有很强的清除作用，清除率可分别达到74.20% 和87.32%。
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| 关 键 词： | 黄粉虫蛋白 抗氧化肽 分离纯化 |
| 收稿时间： | 2008-12-17 |
Separation and Purification of Antioxidant Peptides from Yellow Mealworm |
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| ZHANG Li-sha,ZHANG Jian-xin,OU Xiao-feng,ZHANG Li-jia. Separation and Purification of Antioxidant Peptides from Yellow Mealworm[J]. Food Science, 2009, 30(22): 180-183. DOI: 10.7506/spkx1002-6300-200922040 |
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| Authors: | ZHANG Li-sha ZHANG Jian-xin OU Xiao-feng ZHANG Li-jia |
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| Affiliation: | College of Food Science and Engineering, Northwest A＆F University, Yangling 712100, China |
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| Abstract: | In order to prepare antioxidant peptides from yellow mealworm, yellow mealworm protein powder was hydrolyzed through dual enzyme system and hydrolysates were purified using Sephadex G-25 and ion-exchange column chromatography. The distribution of molecular weight and antioxidant capability of each component were determined. Meanwhile, amino acid composition of the optimal antioxidant peptide was also analyzed. Results indicated that antioxidant peptides from yellow mealworm exhibited strong scavenging capability to hydroxyl free radicals and superoxide free radicals with the scavenging rates reaching 74.20 % and 87.32 %, respectively. |
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| Keywords: | yellow mealworm protein antioxidant peptide separation and purification |
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