| 气相色谱微池电子捕获检测器测定蜂蜡中6种杀螨剂药物残留 |
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| 引用本文： | 陈 芳,李 丹,张金振,黄京平,赵 静,吴黎明. 气相色谱微池电子捕获检测器测定蜂蜡中6种杀螨剂药物残留[J]. 食品科学, 2010, 31(18): 244-247. DOI: 10.7506/spkx1002-6630-201018056 |
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| 作者姓名： | 陈 芳 李 丹 张金振 黄京平 赵 静 吴黎明 |
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| 作者单位： | 1.中国农业科学院蜜蜂研究所，国家农产品加工中心蜂业分中心2.北京城市学院生物医药系 |
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| 基金项目： | 国家现代农业(蜂)产业技术体系建设专项(NYCYTX-43) |
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| 摘 要： | 建立一种准确、快速、简便的蜂蜡中杀螨剂类药物多残留检测技术。样品经恒温水浴液- 液萃取、低温冷冻离心净化后用气相色谱微池电子捕获检测器测定。对提取溶剂的种类、体积及提取次数进行研究。结果表明：30.0mL 乙腈- 正己烷(V/V，2:1)是最佳提取溶剂。经两次冷冻及高速离心能将大部分的脂类基质去除。该方法的线性范围为0.01～4mg/L，相关系数均大于0.9878。检出限为0.96～4.69μg/kg，定量限为2.56～13.12μg/kg。该方法在3 个不同浓度水平的添加均有很好的回收率(83.8%～110.4%)，相对标准偏差为1.70%～6.60%。
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| 关 键 词： | 杀螨剂 蜂蜡 气相色谱微池电子辅获检测器 |
| 收稿时间： | 2010-02-23 |
Determination of Six Acaricide Residues in Beeswax by Gas Chromatography Coupled with a Micro-electron Capture Detector |
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| CHEN Fang,LI Dan,ZHANG Jin-zhen,HUANG Jing-ping,ZHAO Jing,WU Li-ming. Determination of Six Acaricide Residues in Beeswax by Gas Chromatography Coupled with a Micro-electron Capture Detector[J]. Food Science, 2010, 31(18): 244-247. DOI: 10.7506/spkx1002-6630-201018056 |
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| Authors: | CHEN Fang LI Dan ZHANG Jin-zhen HUANG Jing-ping ZHAO Jing WU Li-ming |
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| Affiliation: | 1. Apiculture Sub-Centers, National Agricultural Products Processing Center, Bee Research Institute, Chinese Academy of AgriculturalSciences, Beijing 100093, China；2. Biological Medicine Department, Beijing City University, Beijing 100083, China |
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| Abstract: | An accurate, fast and convenient method for simultaneous determination of 6 acaricide residues in beeswax by gas chromatography coupled with a micro-electron capture detector (GC-MECD) was established. The sample was saturated with hexane and acetonitrile in water bath with constant temperature. After frozen and centrifugation at 10000 r/min, the extract was analyzed by GC-MECD. Effects of extraction solvent, solvent volume and extraction times on extraction efficiency were explored. The best extraction solvent was 30.0 mL of acetonitrile-hexane (V:V, 2:1). Twice freezing and high speed centrifugation could remove the interference of lipid matrix. This method exhibited an excellent linear relationship over the range of 0.01 to 4.0 mg/L with a correlation coefficient of larger than 0.9878. The limits of detection (LOD) for the 6 acaricides varied from 0.96 to 4.69 μg/kg. The average recovery rates of these analytes spiked at three different levels ranged from 83.8% to110.4%, and the relative standard deviations (RSD) from 1.70% to 6.60%. |
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| Keywords: | acaricide beeswax gas chromatography coupled with a micro-electron capture detector (GC-MECD) |
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