| 樟叶木脂素的提取纯化及其抗氧化性、抗癌活性 |
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| 引用本文： | 周海旭,苏同超,李姝,冉军舰,李波,高晗,余梦薇,薛静丽,李婧瑜,李晓晴,李忠海. 樟叶木脂素的提取纯化及其抗氧化性、抗癌活性[J]. 食品工业科技, 2021, 42(13): 170-178. DOI: 10.13386/j.issn1002-0306.2020080306 |
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| 作者姓名： | 周海旭 苏同超 李姝 冉军舰 李波 高晗 余梦薇 薛静丽 李婧瑜 李晓晴 李忠海 |
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| 作者单位： | 1.河南科技学院食品学院,河南新乡 4530032.中南林业科技大学食品科学与工程学院,湖南长沙 410004 |
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| 基金项目： | 国家科技支撑计划（2012BAC01B07-2）;河南省科技攻关项目（202102310143,202102310616）;2017年度高层次人才科研项目（2017020）;2018年度大学生“百农英才”创新项目（BNYC2018-2-36）;河南科技学院2019年大学生创新创业训练计划项目（2019CX025）;河南省高校重点科研项目（21A550008） |
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| 摘 要： | 为了优化樟叶木脂素超声波技术提取条件,探究其抗氧化性、抗肝癌活性,本文通过单因素实验考察各个因素对木脂素提取量的影响,利用响应面试验优化提取工艺并建立相应的数学模型.粗品经过浓缩、萃取、柱色谱等步骤进行分离纯化;紫外色谱、红外光谱、质谱、核磁共振等技术进行结构鉴定.利用高效液相谱(High Efficiency Liq...
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| 关 键 词： | 樟树叶 木脂素 响应面 纯化 抗氧化 细胞毒性 |
| 收稿时间： | 2020-09-01 |
Extraction and Purification of Lignans from Cinnamomum camphora Leaves and Its Antioxidant Activity and Anticancer Activity |
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| ZHOU Haixu,SU Tongchao,LI Shu,RAN Junjian,LI Bo,GAO Han,YU Mengwei,XUE Jingli,LI Jingyu,LI Xiaoqing,LI Zhonghai. Extraction and Purification of Lignans from Cinnamomum camphora Leaves and Its Antioxidant Activity and Anticancer Activity[J]. Science and Technology of Food Industry, 2021, 42(13): 170-178. DOI: 10.13386/j.issn1002-0306.2020080306 |
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| Authors: | ZHOU Haixu SU Tongchao LI Shu RAN Junjian LI Bo GAO Han YU Mengwei XUE Jingli LI Jingyu LI Xiaoqing LI Zhonghai |
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| Affiliation: | 1.Scool of Food Science and Technology, Henan Institute of Science and Technology, Xinxiang 453003, China2.College of Food Science and Engineering, Central South University of Forestry and Technology, Changsha 410004, China |
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| Abstract: | The aim of this study was to evaluate the antioxidant and anticancer activities of lignans from Cinnamomum camphora leaves. Response surface methodology (RSM) was used to optimize ultrasonic wave assisted extraction of lignans based on single factor tests and establish the corresponding mathematical model. The lignans were separated and purified by the steps of concentration, extraction, chromatography-spectrum and so on. The structure was identified by ultraviolet chromatography, infrared spectroscopy, mass spectrometry and nuclear magnetia resonance.The purity of the compounds was determined using HPLC technique and they were used for further experiments. The antioxidant activity of lignans was studied in vitro by scavenging activity against DPPH· and ·OH. The effects of lignans on HepG2 liver cancer cells and human umbilical vein endothelial cell (HUVEC) were compared by MTS assay. The results were as follows: Liquid-to-solid ratio 1cells and human umbilical vein endothelial cell (HUVEC) were compared by MTS assay. The results were as follows: Liquid-to-solid ratio 1:22 g/mL, ultrasonic time 3 min, ethanol concentration 60% and extraction time 78 min, the yield of lignans was 45.31 mg/g. The purity of sesamin and pinoresinol-β-D-glucaside was 94.84%, 90.14% respectively. And the lignans had strong scavenging activity against DPPH· and·OH. Among them, the half inhibitory concentration(IC50) of pinoresinol-β-D-glucaside for scavenging DPPH free radical and hydroxyl free radical was 0.31 mg/mL and 0.71 mg/mL, respectively; the IC50 of sesamin for scavenging DPPH free radical and hydroxyl radical was 0.55 mg/mL and 0.94 mg/mL, respectively. Anticancer activity experiments showed that sesamin had stronger inhibition against HepG2 and HUVEC than pinoresinol-β-D-glucaside. The extraction process by RSM was reliable and the optimal conditions were achieved to get sesamin and pinoresinol-β-D-glucaside, and they had antioxidant and anticancer activities. |
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