| 快速热激处理对甘薯愈伤层形成及代谢的影响 |
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| 引用本文： | 辛奇,孙洁,冯欣欣,赵泽众,刘帮迪,江丽华,郝光飞. 快速热激处理对甘薯愈伤层形成及代谢的影响[J]. 食品科学, 2022, 43(23): 228-238. DOI: 10.7506/spkx1002-6630-20211111-132 |
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| 作者姓名： | 辛奇 孙洁 冯欣欣 赵泽众 刘帮迪 江丽华 郝光飞 |
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| 作者单位： | （1.农业农村部规划设计研究院，北京 100125；2.河北工程大学生命科学与食品工程学院，河北 邯郸 056038；3.农业农村部产地初加工重点实验室，北京 100121） |
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| 基金项目： | “十三五”国家重点研发计划重点专项（2017YFD0401305） |
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| 摘 要： | 为探究快速热激处理（rapid heat treatment，RHT）对促进采后甘薯块根愈伤的影响及机理，本研究以‘大叶红’甘薯为材料，经人工损伤处理后，以传统愈伤处理（35 ℃、2 d，CK＋）和未愈伤处理（CK－）为对照，采用65 ℃热空气对甘薯块根热激15 min，于13 ℃下贮藏7 d，观察愈伤期间甘薯块根伤口处木质素和软木脂的沉积情况；同时，测定块根伤口处组织活性氧代谢中间产物含量和相关酶活力；此外，测定抗氧化活性以及苯丙烷代谢途径关键酶活力和木质素、酚类物质含量。结果表明，RHT能有效促进甘薯块根伤口处木质素和软木脂沉积，与传统愈伤处理效果相近，愈伤并贮藏6 d后块根的木质素和软木脂沉积厚度显著高于对照组（P＜0.05）。RHT处理能显著促进H2O2、·OH和O2－·的快速积累，诱导超氧化物歧化酶、过氧化氢酶、抗坏血酸过氧化物酶、过氧化物酶和多酚氧化酶活力上升，显著提高愈伤期间甘薯愈伤组织的1,1-二苯基-2-三硝基苯肼和2,2-联氮-二(3-乙基-苯并噻唑-6-磺酸)阳离子自由基清除能力（P＜0.05）。RHT还能诱导愈伤期间苯丙氨酸解氨酶、肉桂酸-4-羟基化酶、4-香豆酸辅酶A连接酶和肉桂醇脱氢酶活力上升并提前达到峰值，促进总酚、芦丁和木质素的积累。此外，RHT还显著增加了块根愈伤组织中新绿原酸、儿茶素、绿原酸和表儿茶素含量。综上，RHT不仅可以通过促进活性氧成分的积累和抗氧化活性上升来维持愈伤组织中活性氧的动态平衡，还能激活苯丙烷代谢途径中关键酶，产生大量的次生代谢产物，加速木质素和软木脂沉积，从而促进甘薯块根伤口的快速愈合。
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| 关 键 词： | 快速热激处理；甘薯；愈伤；活性氧代谢；苯丙烷代谢 |
Effect of Rapid Heat Treatment on Wound Healing and Metabolic Mechanism in Sweet Potato |
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| XIN Qi,SUN Jie,FENG Xinxin,ZHAO Zezhong,LIU Bangdi,JIANG Lihua,HAO Guangfei. Effect of Rapid Heat Treatment on Wound Healing and Metabolic Mechanism in Sweet Potato[J]. Food Science, 2022, 43(23): 228-238. DOI: 10.7506/spkx1002-6630-20211111-132 |
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| Authors: | XIN Qi SUN Jie FENG Xinxin ZHAO Zezhong LIU Bangdi JIANG Lihua HAO Guangfei |
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| Affiliation: | (1. Academy of Agricultural Planning and Engineering, Ministry of Agriculture and Rural Affairs, Beijing 100125, China; 2. College of Life Science and Food Engineering, Hebei University of Technology, Handan 056038, China; 3. Key Laboratory of Agro-products Primary Processing, Ministry of Agriculture and Rural Affairs, Beijing 100121, China) |
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| Abstract: | In order to explore the effect and mechanism of rapid heat treatment (RHT) on promoting callus formation in postharvest sweet potato tubers, ‘Dayehong’ sweet potatoes were artificially wounded and divided into a positive control group, a negative control group, and an RHT group. The positive control group was subjected to traditional heat treatment (35 ℃ for two days), and the negative control group was not subjected to any heat treatment. The RHT group was subjected to heat treatment at 65 ℃ for 15 min. All groups were stored at 13 ℃. The experimental period was seven days, consisting of heat treatment and storage. Lignin and suberin deposition at the wound site were observed during wound healing. The intermediate products and enzyme activities in reactive oxygen species (ROS) metabolism were determined, as well as antioxidant activity and the key enzyme activities in the phenylpropane metabolism pathway and the contents of lignin and phenols. The results showed that RHT could effectively promote lignin and suberin deposition at the wound site, and its effect was similar to that of the positive control. On the 6th day of the experiment, the thickness of lignin and suberin deposition was significantly higher than that in the control groups (P < 0.05). RHT treatment significantly promoted the rapid accumulation of H2O2, hydroxyl radical and superoxide anion radical contents, induced the activities of superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX), peroxidase (POD) and polyphenol oxidase (PPO), and significantly increased the scavenging capacity against (1,1-dipheny1-2-picryl-hydrazyl (DPPH) and 2,2’-azinobis-(3-ethylbenzthiazoline-6-sulphonate) (ABTS) cation radical of sweet potato callus during wound healing (P < 0.05). RHT could also induce the increase in phenylalanine ammonia lyase (PAL), cinnamic acid-4-hydroxylase (C4H), 4-coumaryl coenzyme A ligase (4CL) and cinnamyl alcohol dehydrogenase (CAD) activities and make them reach their peak earlier during wound healing, and promoted the accumulation of total phenols, flavonoids and lignin.?Meanwhile, RHT significantly increased the contents of neochlorogenic acid, catechin, chlorogenic acid and epicatechin in sweet potato callus. In conclusion, RHT could not only maintain the dynamic balance of ROS in callus by stimulating the accumulation of ROS and increasing antioxidant activity, but also activate the key enzymes in the phenylpropane metabolic pathway to produce a large number of secondary metabolites and accelerate the deposition of lignin and suberin, thereby promoting rapid wound healing in sweet potato tubers. |
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| Keywords: | rapid heat treatment sweet potato wound healing reactive oxygen species metabolism phenylpropane metabolism, |
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