| 鹿皮胶多糖对酒精性肝损伤的保护作用 |
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| 引用本文： | 刘志洋,孙琪瑶,宫世平,徐建舒,王亚慧,张铭泽,于悦. 鹿皮胶多糖对酒精性肝损伤的保护作用[J]. 食品工业科技, 2021, 42(23): 334-340. DOI: 10.13386/j.issn1002-0306.2021010195 |
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| 作者姓名： | 刘志洋 孙琪瑶 宫世平 徐建舒 王亚慧 张铭泽 于悦 |
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| 作者单位： | 1.长春科技学院生命科学学院，吉林长春 1306002.长春信息技术职业学院科技处，吉林长春 1301033.长春海关技术中心，吉林长春 1300624.吉林农业大学食品科学与工程学院，吉林长春 130118 |
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| 摘 要： | 目的:探究鹿皮胶多糖对急性酒精性肝损伤的保护作用。方法:采用浸提法提取鹿皮胶粗多糖，使用柱色谱分离纯化得到鹿皮胶纯多糖，应用柱色谱、红外光谱及理化学反应实验对其理化性质进行鉴定。通过测定小鼠血清中谷草转氨酶(AST)、谷丙转氨酶 (ALT)、谷胱甘肽过氧化酶(GSH-Px)、超氧化物歧化酶(SOD)活性和丙二醛(MDA)含量及肝匀浆上清液中GSH-Px、SOD活性和MDA含量，观察小鼠肝组织切片，以探究其对急性酒精性肝损伤的保护作用。结果:鹿皮胶多糖属于吡喃糖结构的酸性多糖，其形态为白色絮状，分子量为41.2 kDa，含量达86.71%，无淀粉、酚类、蛋白质、氨基酸及还原糖残基。动物实验结果显示，与模型组比较，鹿皮胶多糖中、高剂量组可显著提高小鼠血清中SOD、GSH-Px活性(P<0.05)，降低ALT、AST活性和MDA含量(P<0.05)；同时可显著提高肝组织匀浆上清液中SOD、GSH-Px活性(P<0.05)，显著降低MDA含量(P<0.05)。结论:鹿皮胶多糖对急性酒精性肝损伤小鼠表现出明显的保护作用。
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| 关 键 词： | 鹿皮胶 多糖 理化性质 酒精性肝损伤 |
| 收稿时间： | 2021-01-25 |
Protective Effect of Deer Skin Glue Polysaccharide on Alcoholic Liver Injury |
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| LIU Zhiyang,SUN Qiyao,GONG Shiping,XU Jianshu,WANG Yahui,ZHANG Mingze,YU Yue. Protective Effect of Deer Skin Glue Polysaccharide on Alcoholic Liver Injury[J]. Science and Technology of Food Industry, 2021, 42(23): 334-340. DOI: 10.13386/j.issn1002-0306.2021010195 |
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| Authors: | LIU Zhiyang SUN Qiyao GONG Shiping XU Jianshu WANG Yahui ZHANG Mingze YU Yue |
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| Affiliation: | 1.College of Life Sciences, Changchun Sci-Tech University, Changchun 130600, China2.Technology Department, Changchun Vocational College of Information Technology, Changchun 130103, China3.Changchun Customs Technology Center Changchun, Changchun 130062, China4.College of Food Science and Engineering, Jilin Agricultural University, Changchun 130118, China |
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| Abstract: | Objective: To explore the protective effect of deer skin gum polysaccharide on acute alcoholic liver injury. Methods: The crude deer skin gum polysaccharide was extracted by extraction method, and purified by column chromatography to obtain pure deer skin gum polysaccharide, and its physical and chemical properties were identified by column chromatography, infrared spectroscopy and physical and chemical reaction experiments. The content of aspartate aminotransferase (AST), alanine aminotransferase (ALT), glutathione peroxidase (GSH-Px), superoxide dismutase (SOD) and malondialdehyde (MDA) in mouse serum and the activity of GSH-Px, SOD and the content of MDA in the supernatant of liver homogenate were measured. The liver tissue sections of mice were observed to explore the protective effect of deer skin gum polysaccharide on acute alcoholic liver injury. Results: The deer skin gum polysaccharide was an acid polysaccharide with the pyranose structure. Its shape was white flocculent, with a molecular weight of 41.2 kDa and the purity of 86.71%. It had no starch, phenols, protein, amino acids and reducing sugar residues.The animal experiment results showed that compared with the model group, the deer skin gum polysaccharide middle and high dose groups could significantly increase the SOD and GSH-Px activities in the serum of mice (P<0.05), and reduce the ALT, AST activities and MDA content (P<0.05). At the same time, it could significantly increase the activity of SOD and GSH-Px in the supernatant of liver tissue homogenate (P<0.05), and significantly reduce the content of MDA (P<0.05). Conclusion: Deer skin gum polysaccharide has obvious protective effect on mice with acute alcoholic liver injury. |
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