| 乡村自然发酵豆腐乳的真菌多样性研究 |
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| 引用本文： | 巫婷玉,徐芳玲,叶蔚歆,虞春华,马嫚,陈晔. 乡村自然发酵豆腐乳的真菌多样性研究[J]. 食品工业科技, 2021, 42(18): 112-118. DOI: 10.13386/j.issn1002-0306.2020120063 |
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| 作者姓名： | 巫婷玉 徐芳玲 叶蔚歆 虞春华 马嫚 陈晔 |
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| 作者单位： | 1.江西医学高等专科学校基础医学部,江西上饶 3340002.九江学院药学与生命科学学院,江西九江 3320003.广东省农业科学院,广东广州 510000 |
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| 基金项目： | 江西省教育厅科技项目（181173） |
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| 摘 要： | 为探究乡村豆腐乳内真菌的多样性,本研究以江西上饶市乡村自然发酵豆腐乳为对象,采用稀释平板培养方法,结合形态特征和LSU序列测序进行菌株鉴定、研究自然发酵豆腐乳真菌多样性.结果表明:从上饶市八个采样地24份豆腐乳样品中分离出277株菌株,鉴定出9个属26个种.根据分离频率,青霉属Penicillium(29.04％)、曲...
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| 关 键 词： | 发酵豆腐乳 真菌 优势属 多样性 相似性 |
| 收稿时间： | 2020-12-09 |
Fungal Diversity of Natural Fermented Soybean Curd in Rural Areas |
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| WU Tingyu,XU Fangling,YE Weiyin,YU Chunhua,MA Man,CHEN Ye. Fungal Diversity of Natural Fermented Soybean Curd in Rural Areas[J]. Science and Technology of Food Industry, 2021, 42(18): 112-118. DOI: 10.13386/j.issn1002-0306.2020120063 |
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| Authors: | WU Tingyu XU Fangling YE Weiyin YU Chunhua MA Man CHEN Ye |
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| Affiliation: | 1.Department of Basic Medicine, Jiangxi Medical College, Shangrao 334000, China2.School of Pharmacy and Life Sciences, Jiujiang University, Jiujiang 332000, China3.Guangdong Academy of Agricultural Sciences, Guangzhou 510000, China |
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| Abstract: | In order to explore the diversity of fungi in rural fermented bean curd, in this study, natural fermented bean curd in rural areas of Shangrao city, Jiangxi province was taken as the object. Strain identification and fungal diversity of natural fermented bean curd were conducted by dilution plate culture method, combined with morphological characteristics and LSU sequence sequencing. The results showed that 277 strains were isolated from 24 samples of fermented bean curd from eight sampling sites in Shangrao, and 26 species from 9 genera were identified. According to the separation frequency, Penicillium (29.04%), Aspergillus (24.65%), Talaromyces (22.88%) and Fusarium (10.57%) were the dominant genus of all the isolates, and there were significant differences among the dominant genera from different sources. The results of diversity index (H) showed that the diversity index (H=1.92) around Shitang in Yanshan County was the highest and the flora richness was high, while the diversity index (H=0.74) in Hongxing living area of Dexing city was the lowest and the flora richness was low. The results of similarity index (Cj) showed that the similarity coefficient of the fungi in fermented bean curd around Shitang in Yanshan County and Xiaokang city Anli community of Guangfeng area was 1.00, and the similarity degree of the fungi in the fermented bean curd was relatively high, while the similarity degree of the fungi in Wenshan Village of Zixi Township and Zhangshu Town of Yushan County was extremely low, with the similarity coefficient of 0.29. The results would provide a theoretical basis for improving the quality and safety of fermented bean curd. |
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