| 点柄乳牛肝菌多糖的结构表征及其免疫调节活性的研究 |
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| 引用本文： | 祁嘉仪,曾冉华,陈忠正,林晓蓉,张玉婷,吴吉莉,高雄,李斌. 点柄乳牛肝菌多糖的结构表征及其免疫调节活性的研究[J]. 食品工业科技, 2021, 42(24): 94-103. DOI: 10.13386/j.issn1002-0306.2021040309 |
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| 作者姓名： | 祁嘉仪 曾冉华 陈忠正 林晓蓉 张玉婷 吴吉莉 高雄 李斌 |
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| 作者单位： | 1.华南农业大学食品学院, 广东广州 5106422.广东省科学院微生物研究所, 华南应用微生物国家重点实验室,广东省微生物安全与健康重点实验室, 广东广州 510070 |
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| 基金项目： | 广东省科学院建设国内一流研究机构行动专项资金项目（2020GDASYL-20200103023）；广东省基础与应用基础研究基金（2019A1515110817） |
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| 摘 要： | 为探究点柄乳牛肝菌多糖的结构特性和免疫调节活性，本研究以点柄乳牛肝菌为材料，经水提醇沉、除蛋白、柱层析分离得到SGP3-1和SGP3-2两个多糖纯化组分。利用高效渗透凝胶色谱（HPGPC）、高效液相色谱（HPLC）、傅里叶红外（FT-IR）、核磁共振波谱（NMR）、X-射线衍射（XRD）等方法表征，结果表明:SGP3-1和SGP3-2的重均分子量（Mw）分别为170.48和14.52 kDa，二者均是无定形态，不含三螺旋结构，SGP3-1以α-构型糖基为主，单糖组成以葡萄糖（55.47%）、甘露糖（20.63%）及半乳糖（14.04%）为主；而SGP3-2以β-构型糖基为主，单糖组成以葡萄糖（82.43%）为主。小鼠单核巨噬细胞RAW264.7体外实验结果表明，SGP3-1和SGP3-2在5~40 μg/mL浓度范围内无细胞毒性，能够增加RAW264.7细胞吞噬活性，均可通过激活丝裂原活化蛋白激酶（MAPKs）、磷脂酰肌醇-3-激酶（PI3K）/丝氨酸/苏氨酸激酶（Akt）及核因子-κB（NF-κB）信号通路，促进一氧化氮（NO）、白细胞介素-6（IL-6）及肿瘤坏死因子-α（TNF-α）产生，从而发挥免疫调节作用，且SGP3-2的免疫调节活性强于SGP3-1，表明SGP3-1和SGP3-2可用于开发功能性食品，作为免疫力低下人群的膳食补充剂，为点柄乳牛肝菌的进一步开发提供理论依据和研究基础。
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| 关 键 词： | 点柄乳牛肝菌 多糖 结构特征 免疫活性 RAW264.7细胞 |
| 收稿时间： | 2021-04-29 |
Structural Characterization and Immunomodulatory Activities of Polysaccharides from Suillus granulatus |
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| QI Jiayi,ZENG Ranhua,CHEN Zhongzheng,LIN Xiaorong,ZHANG Yuting,WU Jili,GAO Xiong,LI Bin. Structural Characterization and Immunomodulatory Activities of Polysaccharides from Suillus granulatus[J]. Science and Technology of Food Industry, 2021, 42(24): 94-103. DOI: 10.13386/j.issn1002-0306.2021040309 |
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| Authors: | QI Jiayi ZENG Ranhua CHEN Zhongzheng LIN Xiaorong ZHANG Yuting WU Jili GAO Xiong LI Bin |
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| Affiliation: | 1.College of Food Science, South China Agricultural University, Guangzhou 510642, China2.Institute of Microbiology, Guangdong Academy of Sciences, State Key Laboratory of Applied Microbiology Southern China, Guangdong Provincial Key Laboratory of Microbial Safety and Health, Guangzhou 510070, China |
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| Abstract: | The present study was aimed to investigate the structural characteristics and immunomodulatory activity of Suillus granulatus polysaccharides. Two polysaccharide fractions(SGP3-1 and SGP3-2) were purified from the fruiting bodies of Suillus granulatus by water extraction and alcohol precipitation, deproteinization, and column chromatography separation. The physicochemical properties of SGP3-1 and SGP3-2 were characterized by high-performance gel permeation chromatography(HPGPC), high-performance liquid chromatography(HPLC), Fourier transform infrared spectroscopy(FT-IR), nuclear magnetic resonance spectroscopy(NMR), X-ray diffraction(XRD), etc. The results showed that the weight-average molecular weight of SGP3-1 and SGP3-2 were 170.48 and 14.52 kDa, respectively. Both SGP3-1 and SGP3-2 were low-crystallinity amorphous polymers, and had no triple-helix conformation. Additionally, SGP3-1 characterized by α-type glycosidic linkage was mainly composed glucose(55.47%), mannose(20.63%) and galactose(14.04%), while SGP3-2 characterized by β-type glycosidic linkage was mainly composed of glucose(82.43%). The results from RAW264.7 cells in vitro suggested that SGP3-1 and SGP3-2 showed no cytotoxicity in the concentration range from 5 to 40 μg/mL. Moreover, SGP3-1 and SGP3-2 could significantly enhance the phagocytic activity and promote the production of nitric oxide(NO), interleukin-6(IL-6), and tumor necrosis factor-α(TNF-α) through activating mitogen-activated protein kinases(MAPKs), phosphatidylinositol-3-kinase(PI3K)/Akt, and nuclear factor-κB(NF-κB) signaling pathways. Furthermore, SGP3-2 exhibited better immunomodulatory activity than SGP3-1. It showed that SGP3-1 and SGP3-2 could be used to develop functional foods, as dietary supplements for people with low immunity, and provide a theoretical basis and research basis for the further development of Suillus granulatus. |
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