| 基于UPLC-Q/Orbitrap HRMS多目标快速筛查鱼肉中30种蛋白同化激素及糖皮质激素 |
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| 引用本文： | 郭添荣,吴文林,张崟,万渝平,叶梅,陈代伟,黄霞,张龙翼. 基于UPLC-Q/Orbitrap HRMS多目标快速筛查鱼肉中30种蛋白同化激素及糖皮质激素[J]. 食品科学, 2022, 43(4): 321-330. DOI: 10.7506/spkx1002-6630-20210203-047 |
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| 作者姓名： | 郭添荣 吴文林 张崟 万渝平 叶梅 陈代伟 黄霞 张龙翼 |
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| 作者单位： | （1.成都市食品检验研究院，四川 成都 611130；2.中国科学院成都生物研究所，四川 成都 610041；3.中国科学院大学，北京 100049；4.成都大学 肉类加工四川省重点实验室，四川 成都 610106） |
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| 基金项目： | 国家市场监督管理总局技术保障专项（2021YJ009）；国家现代农业产业技术体系四川创新团队项目（sccxtd-2021-15）；四川省科技计划应用基础研究项目（21YYJC0962）；成都市技术创新研发项目（2021-YF05-00811-SN） |
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| 摘 要： | 建立基于超高效液相色谱-四极杆/静电场轨道阱高分辨质谱联用技术快速筛查和确证鱼肉中30种蛋白同化激素及糖皮质激素的分析方法.鱼肉样品用80％乙腈溶液(含0.2％甲酸)提取,离心,Oasis PRiME HLB固相萃取柱净化,氮吹后复溶.采用Waters Acquity BEH C18色谱柱(2.1 mm×100mm,1...
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| 关 键 词： | 超高效液相色谱-四极杆/静电场轨道阱高分辨质谱 鱼肉 蛋白同化激素 糖皮质激素 多目标快速筛查 |
Multi-objective Rapid Screening of 30 Protein Assimilation Hormones and Glucocorticoids in Fish by Ultra-high Performance Liquid Chromatography Coupled to Quadrupole/Orbitrap High-Resolution Mass Spectrometry |
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| GUO Tianrong,WU Wenlin,ZHANG Yin,WAN Yuping,YE Mei,CHEN Daiwei,HUANG Xia,ZHANG Longyi. Multi-objective Rapid Screening of 30 Protein Assimilation Hormones and Glucocorticoids in Fish by Ultra-high Performance Liquid Chromatography Coupled to Quadrupole/Orbitrap High-Resolution Mass Spectrometry[J]. Food Science, 2022, 43(4): 321-330. DOI: 10.7506/spkx1002-6630-20210203-047 |
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| Authors: | GUO Tianrong WU Wenlin ZHANG Yin WAN Yuping YE Mei CHEN Daiwei HUANG Xia ZHANG Longyi |
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| Affiliation: | (1. Chengdu Institute of Food Inspection, Chengdu 611130, China; 2. Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China; 3. University of Chinese Academy of Sciences, Beijing 100049, China;4. Key Laboratory of Meat Processing of Sichuan, Chengdu University, Chengdu 610106, China) |
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| Abstract: | A method for rapid screening and identification of 30 protein assimilation hormones and glucocorticoids in fish was established using ultra-high performance liquid chromatography coupled to quadrupole/orbitrap high-resolution mass spectrometry (UPLC-Q/Orbitrap HRMS). The samples were extracted with 80% acetonitrile aqueous solution (containing 0.2% formic acid), centrifuged, purified by solid phase extraction (SPE) on Oasis PRiME HLB column, and dried with nitrogen blow. The residue was re-dissolved. The chromatographic separation was performed on a Waters Acquity BEH C18 (2.1 mm × 100 mm, 1.7 μm) column through gradient elution using a mobile phase consisting of 20 mmol/L ammonium acetate aqueous solution containing 0.1% formic acid (A) and acetonitrile (B). The target analytes were detected in the positive and negative ion mode using a heated electrospray ionization (HESI) source through primary full scan/data-dependent secondary scan (full MS/dd-MS2) monitoring, and quantitated by matrix-matched external standard method. The results showed that good linearity was observed for the 30 hormones in the concentration range of 0.5–100 ng/mL with correlation coefficients (r) greater than 0.995 0. The limits of detection were between 0.2 and 1.0 μg/kg, and the limits of quantification were between 0.5 and 2.0 μg/kg. The recoveries were 69.7%–103.2% with relative standard deviations (RSDs) of 2.3%–9.4% at three spiked levels for four different fishes (turbot, mandarin fish, mullet and grass carp). The proposed method is efficient, accurate and reliable, and is suitable for the rapid multi-objective screening and quantitative analysis of protein assimilation hormones and glucocorticoids in fish. |
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| Keywords: | ultra-high performance liquid chromatography coupled to quadrupole/orbitrap high-resolution mass spectrometry fish protein assimilation hormone glucocorticoid multi-target rapid screening, |
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