| 固载离子液体修饰Fe3O4纳米酶用于H2O2和葡萄糖的检测 |
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| 引用本文： | 杨培昕,喻昌木,杨敏,张荣,汤陆扬,付阳洋,彭黔荣. 固载离子液体修饰Fe3O4纳米酶用于H2O2和葡萄糖的检测[J]. 食品科学, 2021, 42(20): 252-259. DOI: 10.7506/spkx1002-6630-20200924-296 |
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| 作者姓名： | 杨培昕 喻昌木 杨敏 张荣 汤陆扬 付阳洋 彭黔荣 |
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| 作者单位： | （1.贵州大学药学院,贵州 贵阳 550025;2.贵州大学化学与化工学院,贵州 贵阳 550025;3.贵州中烟工业有限责任公司技术中心,贵州 贵阳 550009） |
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| 基金项目： | 国家自然科学基金地区科学基金项目（21562014）;贵州中烟科技项目（黔烟工技[2015]8号） |
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| 摘 要： | 研究SiO2固载离子液体修饰的磁性纳米粒子（Fe3O4@SiO2@IL）制备,及其H2O2酶活性测试。结果表明,Fe3O4@SiO2@IL具有比辣根过氧化物酶更高的催化活性,并能催化H2O2与过氧化物酶底物3,3’,5,5’-四甲基联苯胺形成蓝色产物。因此,开发H2O2比色检测方法,线性范围为4～100 μmol/L,检出限为0.698 μmol/L。该方法还可用于葡萄糖的高灵敏度和选择性检测,线性范围为8～200 μmol/L,检出限为2.39 μmol/L。将该方法用于检测实际样品（如牛奶）中的H2O2,加标回收率为94.0%～107.8%,相对标准偏差为2.24%～3.12%,准确度和精密度较高。具有过氧化物酶活性的Fe3O4@SiO2@IL纳米材料在临床诊断、制药、食品研究等领域具有潜在的应用价值。
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| 关 键 词： | 比色传感器 离子液体 纳米酶 H2O2 葡萄糖 食品检测, |
Immobilized Ionic Liquid Modified Fe3O4 Nanoenzyme for the Detection of Hydrogen Peroxide and Glucose in Foods |
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| YANG Peixin,YU Changmu,YANG Min,ZHANG Rong,TANG Luyang,FU Yangyang,PENG Qianrong. Immobilized Ionic Liquid Modified Fe3O4 Nanoenzyme for the Detection of Hydrogen Peroxide and Glucose in Foods[J]. Food Science, 2021, 42(20): 252-259. DOI: 10.7506/spkx1002-6630-20200924-296 |
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| Authors: | YANG Peixin YU Changmu YANG Min ZHANG Rong TANG Luyang FU Yangyang PENG Qianrong |
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| Affiliation: | (1. School of Pharmaceutical Sciences, Guizhou University, Guiyang 550025, China;2. School of Chemistry and Chemical Engineering, Guizhou University, Guiyang 550025, China;3. Technology Center of China Tobacco Guizhou Industrial Co. Ltd., Guiyang 550009, China) |
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| Abstract: | Silica-immobilized ionic liquid modified magnetic nanoparticles (Fe3O4@SiO2@IL) were prepared and applied as a peroxidase mimic for the detection of H2O2. The results showed that Fe3O4@SiO2@IL had higher catalytic activity than horseradish peroxidase (HRP), and could catalyze the coupling of H2O2 with the peroxidase substrate 3,3’,5,5’-tetramethyl aniline (TMB) to form a blue product. On the basis of this phenomenon, a colorimetric method for H2O2 detection was developed, with a linear range of 4–100 μmol/L and a detection limit of 0.698 μmol/L. The method was also available to sensitively and selectively detect glucose, with a linear range of 8–200 μmol/L and a detection limit of 2.39 μmol/L. The recoveries for this method when applied to detect H2O2 in milk samples ranged from 94.0% to 107.8%, with relative standard deviation (RSD) values of 2.24%–3.12%, showing high accuracy and precision. Therefore, Fe3O4@SiO2@IL nanomaterials with peroxidase mimic activity can be a promising candidate for application in clinical diagnosis, pharmaceutical, food and other fields. |
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| Keywords: | colorimetric sensor ionic liquid nanoenzyme hydrogen peroxide glucose food detection, |
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