| 不同品种绿豆中代谢产物的分离鉴定及代谢机制分析 |
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| 引用本文： | 张丽媛,于英博,赵子莹,于润众,李志江,张东杰. 不同品种绿豆中代谢产物的分离鉴定及代谢机制分析[J]. 食品科学, 2021, 42(16): 169-175. DOI: 10.7506/spkx1002-6630-20200303-044 |
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| 作者姓名： | 张丽媛 于英博 赵子莹 于润众 李志江 张东杰 |
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| 作者单位： | （1.黑龙江八一农垦大学食品学院,黑龙江 大庆 163319;2.黑龙江八一农垦大学电气与信息学院,黑龙江 大庆 163319;3.北大荒现代农业产业技术省级培育协同创新中心,黑龙江 大庆 163319;4.黑龙江省杂粮加工及质量安全工程技术研究中心,黑龙江 大庆 163319;5.国家杂粮工程技术研究中心,黑龙江 大庆 163319） |
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| 基金项目： | 国际合作重点研发项目（2018YFE0206300;2018YFE0206300-09;2018YFE0206300-10）;黑龙江省属本科高校中央支持地方高校改革发展资金优秀青年人才项目（2020YQ16）;黑龙江八一农垦大学三横三纵资助项目（ZRCQC201906）;黑龙江省农垦总局重点研发项目（HKKY190407） |
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| 摘 要： | 利用气相色谱-质谱(gas chromatography-mass spectrometry,GC-MS)分离鉴定不同种类绿豆(大明绿豆和九鲤湖绿豆)中的代谢产物.以代谢组学研究方法为基础,以绿豆为研究对象,先利用甲醇溶液提取极性代谢组分,再用N,O-双(三甲基硅烷基)三氟乙酰胺进行衍生化处理,通过GC-MS进行代谢产...
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| 关 键 词： | 绿豆 代谢物 气相色谱-质谱法 代谢组学 不同品种 |
Separation and Identification of Metabolites and Metabolic Mechanism in Two Cultivars of Mung Bean |
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| ZHANG Liyuan,YU Yingbo,ZHAO Ziying,YU Runzhong,LI Zhijiang,ZHANG Dongjie. Separation and Identification of Metabolites and Metabolic Mechanism in Two Cultivars of Mung Bean[J]. Food Science, 2021, 42(16): 169-175. DOI: 10.7506/spkx1002-6630-20200303-044 |
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| Authors: | ZHANG Liyuan YU Yingbo ZHAO Ziying YU Runzhong LI Zhijiang ZHANG Dongjie |
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| Affiliation: | (1. College of Food Science, Heilongjiang Bayi Agricultural University, Daqing 163319, China;2. College of Electrical and Information, Heilongjiang Bayi Agricultural University, Daqing 163319, China; 3. Cultivating Collaborative Innovation Center for the Beidahuang, Daqing 163319, China; 4. Heilongjiang Engineering Research Center for Coarse Cereals Processing and Quality Safety, Daqing 163319, China; 5. Chinese National Engineering Research Center, Daqing 163319, China) |
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| Abstract: | Based on metabonomics, the metabolites in two mung bean cultivars (Daming and Jiulihu) were isolated and identified by gas chromatography-mass spectrometry (GC-MS). The polar metabolits were extracted with aqueous methanol solution and then derivatized with N,O-bis(trimethylsilyl)trifluoroacetamide (BSTFA) before being analyzed by GC-MS. The results showed that a total 67 metabolites were identified in the mung bean cultivars, 30 of which were found to be common to be both, but at different levels. Ten differential metabolites were identified in Daming mung beans compared with Jiulihu mung beans, and 20 differential metabolites in Jiulihu mung beans compared with Daming mung beans. The metabolic pathways of sugar, fatty acid, amino acid and TCA in Jiulihu mung beans were more active than in Daming mung beans. The dominant metabolites (> 7%) in Daming mung beans were L-norvaline and inositol. The dominant metabolites in Jiulihu mung beans was L-norvaline. We speculated that the difference in the metabolite composition between the two cultivars may be related to the difference in genes, metabolic pathways and other factors. |
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| Keywords: | green bean metabolite gas chromatography-mass spectrometry metabonomics different cultivars, |
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