A Robust Design for Cellular Vehicles of Gold Nanorods for Multimodal Imaging |
| |
| Authors: | Fulvio Ratto Sonia Centi Cinzia Avigo Claudia Borri Francesca Tatini Lucia Cavigli Claudia Kusmic Beatrice Lelli Sarah Lai Stefano Colagrande Francesco Faita Luca Menichetti Roberto Pini |
| |
| Affiliation: | 1. Institute of Applied Physics ‘N. Carrara’, National Research Council Italy, Sesto Fiorentino, (FI), Italy;2. Institute of Clinical Physiology, National Research Council Italy, Pisa, (PI), Italy;3. Department of Experimental and Clinical Biomedical Sciences ‘M. Serio’, University of Florence, Florence, (FI), Italy |
| |
| Abstract: | The pursuit of more selectivity in the delivery of plasmonic particles to tumors is critical before their penetration into clinical applications as the photoacoustic imaging and the photothermal ablation of cancer. As their direct infusion into the bloodstream remains problematic, due to a multitude of biological barriers, the development of alternative approaches is emerging as a new challenge. In this context, the recruitment of homologous tumor‐tropic cells that may serve as Trojan horses stands out as a fascinating possibility. Here, a novel model of gold nanorods is presented that feature a composite shell and undergo efficient and reproducible endocytic uptake from murine macrophages, which is fine‐tunable over a broad range of conditions. These cells preserve their viability and more than 90% of their innate chemotactic behavior in vitro, even with a cargo exceeding 200 000 particles per cell. In addition, we show that these vehicles are detectible by photoacoustic imaging down to concentrations in the order of 1% in whole blood and by clinical X‐ray computed tomography below 10%, which is within the typical fraction of a leukocytic infiltrate in a tumor microenvironment, and may even work as contrast agents for the photothermal ablation of cancer. |
| |
| Keywords: | chemotaxis gold nanoparticles macrophages photoacoustic imaging X‐ray computed tomography |
|
|
