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Selection of Streptococcus thermophilus strains able to produce exopolysaccharides in milk
Authors:Flavio Tidona  Miriam Zago  Milena Corredig  Francesco Locci  Giovanna Contarini  Giorgio Giraffa  Domenico Carminati
Affiliation:1. Consiglio per la Ricerca in Agricoltura e l'Analisi dell'Economia Agraria, Centro di Ricerca per le Produzioni Foraggere e Lattiero‐Casearie (CREA‐FLC), Lodi, Italy;2. Department of Food Science, University of Guelph, Guelph, ON, N1G 3. 2W1, Canada
Abstract:A collection of 139 Streptococcus thermophilus strains was genetically screened by PCR targeting some genes involved in exopolysaccharide (EPS) biosynthesis. Twenty‐nine strains were found to be PCR positive and were grown in skim milk to highlight their ropy character, that is the ability to efficiently thicken the milk cultures and minimise syneresis. Three strains producing a skim milk culture with apparent viscosities of 275 ± 14, 340 ± 21 and 510 ± 42 mPa.s, significantly higher than the average value (135 ± 87.3 mPa.s), were selected. EPS from milk cultures of these three strains was extracted and quantified from 84.4 to 209.2 mg/L.
Keywords:   Streptococcus thermophilus     Starter culture  Texture  Milk gels  Fermented milk
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