| 香菇多糖生物合成代谢相关酶的研究 |
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| 引用本文： | 刘晓翠,田瑾,周芷苒,王晓敏. 香菇多糖生物合成代谢相关酶的研究[J]. 西华大学学报(自然科学版), 2021, 40(5): 90-97. DOI: 10.12198/j.issn.1673-159X.3952 |
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| 作者姓名： | 刘晓翠 田瑾 周芷苒 王晓敏 |
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| 作者单位： | 西华大学食品与生物工程学院, 四川 成都 6100039 |
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| 基金项目： | 教育部春晖计划项目(191634)；食品生物技术重点实验室（重点研究基地）开放课题资助（szjj2017-107） |
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| 摘 要： | 以香菇菌丝体多糖含量及其生物合成相关酶的活性为研究指标,采用单因素实验和正交实验确定液体发酵的最佳条件,优化香菇菌丝体的培养工艺,找到控制菌丝体多糖生物合成代谢的关键酶.结果表明:香菇菌丝体最适碳源为40?g/L葡萄糖,最适氮源为10?g/L牛肉膏,最适发酵时间为5?d,最适pH值和培养温度为7和25?℃.同时在不同发...
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| 关 键 词： | 香菇 多糖 生物合成代谢 酶活性 |
| 收稿时间： | 2021-02-22 |
Study on Enzymes Related to Lentinan Biosynthesis Metabolism |
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| LIU Xiaocui,TIAN Jin,ZHOU Zhiran,WANG Xiaomin. Study on Enzymes Related to Lentinan Biosynthesis Metabolism[J]. Journal of Xihua University(Natural Science Edition), 2021, 40(5): 90-97. DOI: 10.12198/j.issn.1673-159X.3952 |
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| Authors: | LIU Xiaocui TIAN Jin ZHOU Zhiran WANG Xiaomin |
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| Affiliation: | School of Food and Bioengineering, Xihua University, Chengdu 610039 China |
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| Abstract: | In this design, the yield of mycelium polysaccharides and the activity of lentinan-synthesizing enzymes were used as the research indicators. The single factor test was used to obtain the best conditions for liquid fermentation. Then, the best culture process of Letinous edodes mycelia was optimized by orthogonal design and the key enzymes controlling polysaccharide biosynthesis of mycelia were found. The results show that the optimal fermentation time for Lentinus edodes mycelium is 5 days, and the optimal carbon source and nitrogen source are 40 g/L glucose, 10 g/L beef extract, and the optimal culture pH and temperature are 7 and 25 ℃. At the same time, Lentinus edodes mycelium was cultured under different fermentation conditions. Intracellular glucokinase (GK), phosphoglucose mutase (PGM), UDP-glucose pyrophosphorylase (UGP), and phosphoglucose isomerase (PGI) whose activity increased with the increase of polysaccharide production indicating that these five enzymes are related to the biosynthesis of polysaccharides in Lentinus edodes mycelium. |
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