| 高纯胸腺肽α1的制备 |
| |
| 引用本文： | 刘标,汤斌. 高纯胸腺肽α1的制备[J]. 化工学报, 2013, 64(7): 2497-2502. DOI: 10.3969/j.issn.0438-1157.2013.07.026 |
| |
| 作者姓名： | 刘标 汤斌 |
| |
| 作者单位： | 安徽工程大学生物与化学工程学院, 安徽 芜湖 241000 |
| |
| 摘 要： | 为了获得高纯产品,在制备胸腺肽α1(Tα1)工艺中分别对合成、纯化两步骤进行控制。在固相多肽合成过程中,以TBTU/HOBt/DIEA体系作为缩合试剂,在易形成α螺旋和β转角的肽序位置,通过采用HOBt/DIC补投的方法促使偶联反应完全。在分离纯化阶段,结合反相色谱和离子色谱的优势,以反相离子对液相色谱法为主要分离手段,通过多步分离实现纯化。获得的粗肽纯度为67.2%,胸腺肽α1产品纯度达99.5%以上,总收率为17.2%。此法可作为制备高纯多肽药物的有效手段。
|
| 关 键 词： | 胸腺肽α1 固相多肽合成 反相离子对液相色谱 |
| 收稿时间： | 2012-11-07 |
| 修稿时间： | 2013-03-01 |
Preparation of high-purity thymosin α1 |
| |
| LIU Biao , TANG Bin. Preparation of high-purity thymosin α1[J]. Journal of Chemical Industry and Engineering(China), 2013, 64(7): 2497-2502. DOI: 10.3969/j.issn.0438-1157.2013.07.026 |
| |
| Authors: | LIU Biao TANG Bin |
| |
| Affiliation: | College of Biological and Chemical Engineering, Anhui Polytechnic University, Wuhu 241000, Anhui, China |
| |
| Abstract: | The synthesis and purification steps were controlled in preparation of thymosin α1 to obtain the product with high-purity.In the solid-phase peptide synthesis (SPPS)process,TBTU/HOBt/DIEA was employed as the coupling reagent.In the sites where α-helix or β-turn structure formed easily,re-coupling reagent of HOBt/DIC was used to ensure a complete reaction.In the separation and purification process,a multi-step method was employed,in which reversed-phase ion-pair liquid chromatography was used as the main means,combining the advantages of reversed-phase chromatography and ion chromatography.The results showed that through the re-coupling the crude peptide had a purity of 67.2% with the use of TBTU/HOBt/DIEA reagent in SPPS.The purity of the refined thymosin α1 was above 99.5% with a total yield of 17.2% with the reversed-phase ion-pair liquid chromatography.This separation process avoids complex chromatography separation process.The method may be an efficient way for preparation of peptides with high-purity. |
| |
| Keywords: | thymosin α1 solid-phase peptide synthesis reversed-phase ion-pair liquid chromatography |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《化工学报》浏览原始摘要信息 |
|
点击此处可从《化工学报》下载免费的PDF全文 |
|
