| 液相色谱-串联质谱法测定糖果中2-(4-甲氧基苯氧基)-丙酸钠甜味抑制剂 |
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| 引用本文： | 陈桐, 周洪斌, 王现平, 肖震, 陆慧媛, 李平, 沈伟健, 王红. 液相色谱-串联质谱法测定糖果中2-(4-甲氧基苯氧基)-丙酸钠甜味抑制剂[J]. 食品工业科技, 2019, 40(15): 230-234. DOI: 10.13386/j.issn1002-0306.2019.15.038 |
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| 作者姓名： | 陈桐 周洪斌 王现平 肖震 陆慧媛 李平 沈伟健 王红 |
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| 作者单位： | 1. 镇江海关, 江苏镇江 212008;2. 南京海关动植物与食品检测中心, 江苏南京 210001 |
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| 基金项目： | 江苏省青年科技基金项目(BK20161075);2016年度江苏省“六大人才高峰”项目(XCL-146);2018年国家重点研发计划(2018YFF0215605) |
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| 摘 要： | 建立了高效液相色谱-串联质谱法检测糖果中的2-(4-甲氧基苯氧基)-丙酸钠的方法。采用水溶液(pH8.0):乙腈=1.5:1 (V/V)作为提取溶剂,然后用正己烷去除油脂,取水层清液过膜,进行检测。采用电喷雾负离子模式(ESI-)和选择反应监测扫描(SRM)模式,外标法定量。结果表明,该方法在6.25~100 μg/L范围内线性关系良好(相关系数r=0.9998),检出限为8.0 μg/kg,定量限为20.0 μg/kg。回收率为82.7%~90.7%,相对标准偏差为2.5%~4.2%。该方法前处理简单、重现性好、灵敏度高,为糖果中的2-(4-甲氧基苯氧基)-丙酸钠的检测提供一种新的选择。
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| 关 键 词： | 高效液相色谱-串联质谱 糖果 2-(4-甲氧基苯氧基)-丙酸钠 甜味抑制剂 |
| 收稿时间： | 2018-09-21 |
Determination of 2-(4-Methoxyphenoxy)-Propionic Acid Sweetness Inhibitor in Candies by Liquid Chromatography-Tandem Mass Spectrometry |
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| CHEN Tong, ZHOU Hong-bin, WANG Xian-ping, XIAO Zhen, LU Hui-yuan, LI Ping, SHEN Wei-jian, WANG Hong. Determination of 2-(4-Methoxyphenoxy)-Propionic Acid Sweetness Inhibitor in Candies by Liquid Chromatography-Tandem Mass Spectrometry[J]. Science and Technology of Food Industry, 2019, 40(15): 230-234. DOI: 10.13386/j.issn1002-0306.2019.15.038 |
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| Authors: | CHEN Tong ZHOU Hong-bin WANG Xian-ping XIAO Zhen LU Hui-yuan LI Ping SHEN Wei-jian WANG Hong |
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| Affiliation: | 1. Zhenjiang Customs District P. R. of China, Zhenjing 212008, China;2. Animal, Plant and Food Inspection Center, Nanjing Customs District P. R. of China, Nanjing 210001, China |
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| Abstract: | A high performance liquid chromatography-tandem mass spectrometryic(HPLC-MS/MS)method was established for the detection of sodium 2-(4-methoxyphenoxy)-propanoate in candies.The sample was pretreated by using an aqueous solution(pH8.0)∶acetonitrile=1.5∶1(V/V)as an extraction solvent,and then fat was removed by hexane. The clear liquid filtrated with membrane was detected. The analytes were identified by HPLC-MS/MS with the negative electrospray ionization(ESI^-)under selected reaction monitoring(SRM)mode,and analyzed quantitatively by the external standard method. Results showed that the calibration curves were linear in the concentration range of 6.25~100.00 μg/L(correlation coefficients was 0.9998).The limits of detection(LODs)of the sodium 2-(4-methoxyphenoxy)-propanoate was 8.0 μg/kg,and the limits of quantitation(LOQs)was 20.0 μg/kg. The average recoveries were in the range of 82.7%~90.7% with relative standard derivations(RSDs)of 1.2%~2.5%.The method was simple,sensitive and accurate. It provided a new method of the determination of the sodium 2-(4-methoxyphenoxy)-propanoate residue in candies. |
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| Keywords: | high performance liquid chromatography-tandem mass spectrometry(HPLC-MS/MS) candies sodium 2-(4-methoxyphenoxy)-propanoate sweetness inhibitor |
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