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Rapid Self‐Assembly of Macroscale Tissue Constructs at Biphasic Aqueous Interfaces |
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| Authors: | John P. Frampton Brendan M. Leung Eve L. Bingham Sasha Cai Lesher‐Perez Jack D. Wang Hady T. Sarhan Mohamed E. H. El‐Sayed Stephen E. Feinberg Shuichi Takayama |
| Affiliation: | 1. School of Biomedical Engineering, Dalhousie University, Halifax, NS, Canada;2. Department of Biomedical Engineering, University of Michigan, Ann Arbor, MI, USA;3. Biointerfaces Institute, University of Michigan, Ann Arbor, Michigan, USA;4. Department of Oral and Maxillofacial Surgery, University of Michigan, Ann Arbor, MI, USA;5. Department of Macromolecular Science and Engineering, University of Michigan, Ann Arbor, MI, USA |
| Abstract: | An entirely new approach to tissue engineering is presented that uses the interfacial forces between aqueous solutions of phase‐separating polymers to confine cells and promote their assembly into interconnected, macroscopic tissue constructs. This simple and inexpensive general procedure creates free‐standing, centimeter‐scale constructs from cell suspensions at the interface between poly(ethylene glycol) and dextran aqueous two‐phase systems in as little as 2 h. Using this method, skin constructs are produced that integrate with decellularized dermal matrices, on which they differentiate and stratify into skin equivalents. It is demonstrated that the constructs produced by this method have appropriate integrity and mechanical properties for use as in vitro tissue models. |
| Keywords: | 3D culture aqueous two‐phase system interfaces self‐assembly tissue constructs |